Carlson J, Helmby H, Hill A V, Brewster D, Greenwood B M, Wahlgren M
Department of Immunology, Stockholm University, Sweden.
Lancet. 1990 Dec 15;336(8729):1457-60. doi: 10.1016/0140-6736(90)93174-n.
Plasmodium falciparum isolates from 24 Gambian children with cerebral malaria and 57 children with mild forms of the disease were assessed for their ability to form erythrocyte rosettes. All isolates from the children with cerebral malaria were able to form rosettes, whereas those from children with mild forms of the disease did not form rosettes, or had a significantly lower rosetting rate. Plasma of children with cerebral malaria lacked anti-rosetting activity, whereas plasma of children with mild disease could often disrupt rosettes in vitro. A monoclonal antibody to P falciparum histidine rich protein (PfHRP1/KP/KAHRP) disrupted rosettes of many of the isolates in vitro indicating that the rosetting ligand is relatively conserved compared with ligands associated with endothelial cytoadherence. The findings strongly support the hypothesis that erythrocyte rosetting contributes to the pathogenesis of cerebral malaria and suggest that anti-rosetting antibodies protect against cerebral disease.
对来自24名患有脑型疟疾的冈比亚儿童以及57名患有轻度疟疾的儿童的恶性疟原虫分离株进行了形成红细胞玫瑰花结能力的评估。所有来自脑型疟疾儿童的分离株都能够形成玫瑰花结，而来自轻度疟疾儿童的分离株则不能形成玫瑰花结，或者玫瑰花结形成率显著较低。脑型疟疾儿童的血浆缺乏抗玫瑰花结活性，而轻度疾病儿童的血浆通常能够在体外破坏玫瑰花结。一种针对恶性疟原虫富含组氨酸蛋白（PfHRP1/KP/KAHRP）的单克隆抗体在体外破坏了许多分离株的玫瑰花结，这表明与内皮细胞粘附相关的配体相比，玫瑰花结配体相对保守。这些发现有力地支持了红细胞玫瑰花结形成有助于脑型疟疾发病机制的假说，并表明抗玫瑰花结抗体可预防脑部疾病。
