Gilad G M, Gilad V H, Wyatt R J, Tizabi Y
Neuropsychiatry Branch, NIMH Neuroscience Center, St. Elizabeths, Washington, D.C. 20032.
Brain Res. 1990 Aug 20;525(2):335-8. doi: 10.1016/0006-8993(90)90886-g.
The study describes stress-induced changes in high-affinity uptake and release of glutamate by synaptosomal preparations from several regions of rat brain. The results demonstrate that restraint stress can lead to increased glutamate uptake and release in limbic forebrain regions (frontal cortex, hippocampus and septum) but not in the striatum. The increase in glutamate uptake was evident after 30 min of stress. A plateau (140-150% of unhandled controls) was reached after 1 h and was maintained after 4 h of continuous stress. The stress-induced increase in glutamate uptake was observed with glutamate concentrations of up to 10 microM, but not with 500 microM. the results indicate that forebrain glutamatergic terminals are activated by stressful stimuli in a regionally selective manner, and suggest that enhanced high-affinity uptake is important in clearing increased levels of released glutamate.
该研究描述了应激诱导大鼠脑多个区域突触体谷氨酸高亲和力摄取和释放的变化。结果表明，束缚应激可导致边缘前脑区域（额叶皮质、海马体和隔区）谷氨酸摄取和释放增加，但纹状体无此变化。应激30分钟后谷氨酸摄取增加明显。持续应激1小时后达到平台期（未处理对照组的140 - 150%），并在4小时后维持。在谷氨酸浓度高达10微摩尔时观察到应激诱导的谷氨酸摄取增加，但500微摩尔时未观察到。结果表明，前脑谷氨酸能终末以区域选择性方式被应激刺激激活，并提示增强的高亲和力摄取对于清除释放增加的谷氨酸水平很重要。
