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副结核分枝杆菌DNA中限制性片段长度多态性的鉴定

Identification of restriction fragment length polymorphisms in DNA from Mycobacterium paratuberculosis.

作者信息

Whipple D, Kapke P, Vary C

机构信息

Leptospirosis/Mycobacteriosis Research Unit, U.S. Department of Agriculture, Ames, Iowa 50010.

出版信息

J Clin Microbiol. 1990 Nov;28(11):2561-4. doi: 10.1128/jcm.28.11.2561-2564.1990.

DOI:10.1128/jcm.28.11.2561-2564.1990
PMID:1979332
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC268225/
Abstract

DNAs from 34 mycobactin-dependent isolates of Mycobacterium paratuberculosis and 1 isolate of M. paratuberculosis 18 were digested with four restriction endonucleases. Southern hybridization experiments were performed with a 32P-labeled oligonucleotide DNA probe derived from the sequence of IS900, an insertion sequence present in 15 to 20 copies per M. paratuberculosis chromosome. The probe hybridized with DNA from each of the mycobactin-dependent isolates, and restriction fragment length polymorphisms were detected among the isolates with each restriction endonuclease used. Restriction fragment length polymorphism analysis may permit identification of various strains of M. paratuberculosis, which has not been possible with other techniques.

摘要

用四种限制性内切酶消化34株副结核分枝杆菌的铁转运蛋白依赖型菌株以及1株副结核分枝杆菌18的DNA。采用源自IS900序列的32P标记寡核苷酸DNA探针进行Southern杂交实验，IS900是一种插入序列，每个副结核分枝杆菌染色体中有15至20个拷贝。该探针与每株铁转运蛋白依赖型菌株的DNA杂交，并且在用每种限制性内切酶处理的菌株中检测到了限制性片段长度多态性。限制性片段长度多态性分析可能有助于鉴定副结核分枝杆菌的各种菌株，而这是其他技术无法做到的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c27/268225/aa082fc4c037/jcm00059-0195-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c27/268225/aa082fc4c037/jcm00059-0195-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c27/268225/aa082fc4c037/jcm00059-0195-a.jpg

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