Department of Chemical Engineering, University of Washington, Seattle, WA 98195, USA.
J Biomater Sci Polym Ed. 2009;20(13):1845-59. doi: 10.1163/156856208X386444.
Zwitterionic hydrogels, including poly(sulfobetaine methacrylate) (polySBMA) and poly(carboxybetaine methacrylate) (polyCBMA), and co-polymeric hydrogels of CBMA and 2-hydroxyethyl methacrylate (HEMA) (poly(CBMA-co-HEMA)) were prepared. Their in vitro and in vivo properties were evaluated and compared with those of polyHEMA hydrogels. Bovine aortic endothelial cells (BAECs) were incubated with zwitterionic and polyHEMA hydrogels to evaluate their bioadhesion properties. Both polySBMA and polyCBMA hydrogels were found to be non-cytotoxic and their endotoxin levels were found to be acceptable for in vivo implantation. Results from in vivo subcutaneous implantation showed reduced cell attachment to the surfaces of polySBMA and poly(CBMA-co-HEMA) hydrogels after one-week implantation as compared with polyHEMA hydrogels. After a 4-week implantation, capsules with higher vascularities surrounding the two zwitterionic hydrogels were found. However, polyHEMA, polySBMA and poly(CBMA-co-HEMA) hydrogels showed similar capsule thicknesses and similar numbers of attached foreign body giant cells (FBGCs). In this work, zwitterionic hydrogels demonstrate healing and integration comparable to polyHEMA hydrogels, but with improved vascularity. These zwitterionic hydrogels are promising alternatives to polyHEMA hydrogels as implantable materials.
两性离子水凝胶，包括聚（磺酸甜菜碱甲基丙烯酸酯）（polySBMA）和聚（羧酸甜菜碱甲基丙烯酸酯）（polyCBMA），以及 CBMA 和 2-羟乙基甲基丙烯酸酯（HEMA）的共聚物水凝胶（poly（CBMA-co-HEMA））被制备。评估了它们的体外和体内性质，并与 polyHEMA 水凝胶进行了比较。将牛主动脉内皮细胞（BAEC）与两性离子和 polyHEMA 水凝胶孵育，以评估它们的生物黏附性质。发现聚 SBMA 和聚 CBMA 水凝胶均无细胞毒性，其内毒素水平可接受体内植入。体内皮下植入结果表明，与 polyHEMA 水凝胶相比，植入一周后，polySBMA 和 poly（CBMA-co-HEMA）水凝胶表面的细胞附着减少。在 4 周的植入后，发现围绕着两种两性离子水凝胶的囊泡具有更高的血管化程度。然而，polyHEMA、polySBMA 和 poly（CBMA-co-HEMA）水凝胶的囊泡厚度和附着的异物巨细胞（FBGC）数量相似。在这项工作中，两性离子水凝胶表现出与 polyHEMA 水凝胶相当的愈合和整合能力，但具有更高的血管化程度。这些两性离子水凝胶是作为可植入材料替代 polyHEMA 水凝胶的有前途的选择。
