Department of Chemical Engineering, University of Washington, Box 351750, Seattle, WA 98195, USA.
Biomaterials. 2011 Feb;32(4):961-8. doi: 10.1016/j.biomaterials.2010.09.067. Epub 2010 Oct 20.
We have introduced a dimethacrylate carboxybetaine-based crosslinker that has excellent compatibility with zwitterionic hydrogel systems. Poly(carboxybetaine methacrylate) (pCBMA) hydrogels prepared with the new CBMA crosslinker (CBMAX) result in considerably improved solubility, homogeneity, and mechanical properties (up to 8 MPa compressive modulus) over those prepared with the commercially available N,N'-methylenebis(acrylamide) (MBAA) crosslinker. The zwitterionic nature of the CBMAX crosslinker provides continuity of ordered hydration in the CBMA hydrogel and retains its nonfouling properties. CBMAX-crosslinked CBMA hydrogels had lower cell fouling than MBAA-crosslinked CBMA hydrogels and in fact reduced cell adhesion by about 90% relative to pHEMA hydrogels. Furthermore, unlike pHEMA, CBMA hydrogels are readily functionalizable. Cell adhesion on nonfouling CBMA hydrogels was controlled by cRGD functionalization.
我们引入了一种基于二甲基丙烯酰胺羧基甜菜碱的交联剂，它与两性离子水凝胶系统具有极好的相容性。用新型 CBMA 交联剂 (CBMAX) 制备的聚 (羧基甜菜碱甲基丙烯酰胺) (pCBMA) 水凝胶在溶解度、均一性和机械性能（高达 8 MPa 的压缩模量）方面明显优于用商业 N,N'-亚甲基双丙烯酰胺 (MBAA) 交联剂制备的水凝胶。CBMAX 交联剂的两性离子性质在 CBMA 水凝胶中提供了有序水合的连续性，并保持其抗污性能。CBMAX 交联的 CBMA 水凝胶的细胞污染程度低于 MBAA 交联的 CBMA 水凝胶，实际上相对于 pHEMA 水凝胶降低了约 90%的细胞黏附。此外，与 pHEMA 不同，CBMA 水凝胶易于功能化。细胞在非污染的 CBMA 水凝胶上的黏附通过 cRGD 功能化来控制。
