工程化异源抗原的细胞靶向是基于牛疱疹病毒4的疫苗载体开发的决定性因素。
Cellular targeting of engineered heterologous antigens is a determinant factor for bovine herpesvirus 4-based vaccine vector development.
作者信息
Donofrio Gaetano, Franceschi Valentina, Capocefalo Antonio, Taddei Simone, Sartori Chiara, Bonomini Sabrina, Cavirani Sandro, Cabassi Clotilde S, Flammini Cesidio F
机构信息
Department of Animal Health, Sezione di Malattie Infettive degli Animali, Facoltà di Medicina Veterinaria, University of Parma, Parma, Italy.
出版信息
Clin Vaccine Immunol. 2009 Nov;16(11):1675-86. doi: 10.1128/CVI.00224-09. Epub 2009 Sep 30.
Abstract
In a previous study, an apathogenic strain of bovine herpesvirus 4 (BoHV-4) cloned as a bacterial artificial chromosome and expressing a chimeric peptide (gE2/gD) as a secreted form was described. Recombinant virus-inoculated animals produced antibodies against bovine viral diarrhea virus (BVDV) gE2 and BoHV-1 gD. However, neutralizing antibodies were produced only against BVDV, not against BoHV-1. In the present work a recombinant BoHV-4 expressing a membrane-linked form of gE2/gD chimeric peptide was constructed, and inoculated rabbits produced serum-neutralizing antibodies against both BVDV and BoHV-1. Protein cell sorting and targeting are a very important issue when immunodominant antigens are engineered for recombinant virus vaccine development.
摘要
在之前的一项研究中，描述了一种牛疱疹病毒4（BoHV-4）的无致病性菌株，其作为细菌人工染色体进行克隆，并表达一种作为分泌形式的嵌合肽（gE2/gD）。接种重组病毒的动物产生了针对牛病毒性腹泻病毒（BVDV）gE2和BoHV-1 gD的抗体。然而，仅产生了针对BVDV的中和抗体，而未产生针对BoHV-1的中和抗体。在本研究中，构建了一种表达gE2/gD嵌合肽膜连接形式的重组BoHV-4，接种后的兔子产生了针对BVDV和BoHV-1的血清中和抗体。当为重组病毒疫苗开发设计免疫显性抗原时，蛋白质细胞分选和靶向是一个非常重要的问题。
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