Department of Pharmaceutical Biology, Pharmaceutical Institute, Christian-Albrechts-University of Kiel, Kiel, Germany.
Biomacromolecules. 2009 Nov 9;10(11):2998-3008. doi: 10.1021/bm900501g.
The red seaweed Delesseria sanguinea dominantly populates a large artificial reef in the southwestern Baltic Sea. It contains sulfated polysaccharides (SPs), which exhibit a pharmacological profile indicating anti-inflammatory and anti-skin aging potencies. To establish and optimize an extraction procedure for these SPs and to evaluate the influence of several parameters on their quality, 23 algae batches were harvested over the period of four years and extracted by different methods, resulting in 56 SP batches. Extraction with water at 85 degrees C proved to be superior and led to highly reproducible products with average yields of 11.6 +/- 3.9%, reaching 18% in spring. Their quality was independent of generation form and vitality of the algae. The SPs were identified as sulfated branched xylogalactans (degree of sulfation 0.50 +/- 0.08, mean M(r) 142000). The coextraction of floridean starch turned out to be the only parameter causing any seasonal variability. However, by using water, this concerns solely the yields of the isolated products. Compared to NaOH extracts, the antielastase activity of H(2)O extracted SPs was about twice as strong (IC(50) 0.204 +/- 0.024 microg/mL) and the batch to batch variability was much lower (CV 11.8 vs 28.6%). In conclusion, SPs from Delesseria sanguinea can be isolated in reproducibly high quality by using a specific extraction procedure. Thus, an important prerequisite for a potential economical utilization is fulfilled.
红海草 Delesseria sanguinea 主要分布在波罗的海南部的一个大型人工礁石上。它含有硫酸多糖 (SPs)，具有抗炎和抗皮肤老化的功效。为了建立和优化提取这些 SPs 的方法，并评估几个参数对其质量的影响，我们在四年的时间里从 23 批藻类中收获了 56 批 SPs，采用不同的方法进行了提取，结果表明 85°C 水提取法优越，并产生了高度重现性的产品，平均收率为 11.6±3.9%，春季可达到 18%。其质量与藻类的世代形式和活力无关。SPs 被鉴定为硫酸化支链木半乳聚糖（硫酸化度 0.50±0.08，平均 M(r)142000）。共提取的叶状淀粉是唯一导致季节性变化的参数。然而，通过使用水，这仅涉及到分离产物的收率。与 NaOH 提取物相比，H(2)O 提取的 SPs 的抗弹性蛋白酶活性要强约两倍（IC(50)0.204±0.024μg/mL），批次间的变异性要低得多（CV 11.8%对 28.6%）。总之，使用特定的提取程序可以从 Delesseria sanguinea 中分离出重复性好的高质量 SPs。因此，为潜在的经济利用提供了一个重要的前提条件。
