Wiegand Annette, Magalhães Ana Carolina, Navarro Ricardo Scarparo, Schmidlin Patrick Roger, Rios Daniela, Buzalaf Marilia Afonso Ribeiro, Attin Thomas
Clinic for Preventive Dentistry, Periodontology and Cariology, University of Zürich, Zürich, Switzerland.
Photomed Laser Surg. 2010 Apr;28(2):219-26. doi: 10.1089/pho.2009.2551.
This in vitro study aimed to analyze the influence of carbon dioxide (CO(2)) laser irradiation on the efficacy of titanium tetrafluoride (TiF(4)) and amine fluoride (AmF) in protecting enamel and dentin against erosion.
Bovine enamel and dentin samples were pretreated with carbon dioxide (CO(2)) laser irradiation only (group I), TiF(4) only (1% F, group II), CO(2) laser irradiation before (group III) or through (group IV) TiF(4) application, AmF only (1% F, group V), or CO(2) laser irradiation before (group VI) or through (group VII) AmF application. Controls remained untreated. Ten samples of each group were then subjected to an erosive demineralization and remineralization cycling for 5 days. Enamel and dentin loss were measured profilometrically after pretreatment, 4 cycles (1 day), and 20 cycles (5 days) and statistically analyzed using analysis of variance and Scheffe's post hoc tests. Scanning electron microscopy (SEM) analysis was performed in pretreated but not cycled samples (two samples each group).
After 20 cycles, there was significantly less enamel loss in groups V and IV and significantly less dentin loss in group V only. All other groups were not significantly different from the controls. Lased surfaces (group I) appeared unchanged in the SEM images, although SEM images of enamel but not of dentin showed that CO(2) laser irradiation affected the formation of fluoride precipitates.
AmF decreased enamel and dentin erosion, but CO(2) laser irradiation did not improve its efficacy. TiF(4) showed only a limited capacity to prevent erosion, but CO(2) laser irradiation significantly enhanced its ability to reduce enamel erosion.
本体外研究旨在分析二氧化碳（CO₂）激光照射对四氟化钛（TiF₄）和氟化胺（AmF）保护牙釉质和牙本质免受侵蚀功效的影响。
牛牙釉质和牙本质样本分别仅用二氧化碳（CO₂）激光照射（I组）、仅用TiF₄（1%氟，II组）、在应用TiF₄之前（III组）或期间（IV组）进行CO₂激光照射、仅用AmF（1%氟，V组）、在应用AmF之前（VI组）或期间（VII组）进行CO₂激光照射。对照组不做处理。然后每组十个样本进行为期5天的侵蚀性脱矿和再矿化循环。在预处理后、4个循环（1天）和20个循环（5天）后用轮廓仪测量牙釉质和牙本质的损失，并使用方差分析和谢费事后检验进行统计分析。对预处理但未进行循环的样本（每组两个样本）进行扫描电子显微镜（SEM）分析。
20个循环后，V组和IV组的牙釉质损失显著减少，仅V组的牙本质损失显著减少。所有其他组与对照组无显著差异。在SEM图像中，激光照射的表面（I组）看起来没有变化，尽管牙釉质而非牙本质的SEM图像显示CO₂激光照射影响了氟化物沉淀的形成。
AmF减少了牙釉质和牙本质的侵蚀，但CO₂激光照射并未提高其功效。TiF₄在预防侵蚀方面仅显示出有限的能力，但CO₂激光照射显著增强了其减少牙釉质侵蚀的能力。
