Department of Restorative Dentistry, Federal University of Pará, Belém, Pará, Brazil.
Department of Restorative Dentistry, Sao Paulo State University, Araraquara, São Paulo, Brazil.
Braz Dent J. 2023 May-Jun;34(3):94-100. doi: 10.1590/0103-6440202305325.
This in vitro study evaluated the effect of an experimental varnish containing 20% nano-hydroxyapatite (nHAP) associated with 5% stannous chloride (SnCl2) against erosive-abrasive wear on bovine dentin. Samples of bovine cervical dentin were pre-eroded (0.3% citric acid, pH 2.6 for 10 minutes) and randomized into 4 groups (n=10): Control group - experimental varnish without active ingredient (CG); experimental varnish containing 20% nHAP (nHG); experimental varnish containing 5% SnCl2 (24.800 ppm Sn2+) (SnG); experimental varnish containing 20% nHAP associated with 5% SnCl2 (18.300 ppm Sn2+) (nHSnG). After applying the materials, the erosive-abrasive challenges were performed for five days. Erosive dentin loss and analysis of the pattern of dentinal obliteration were performed by 3D confocal laser microscopy. A one-way ANOVA/Bonferroni test was performed to analyze the data (α=0.05). The SnG and nHSnG experimental groups presented more effectiveness in preventing erosive wear when compared to the other groups (p<0.05). There was no statistically significant difference between the SnG and nHSnG groups (p = 0.731) in tooth structure dentin loss. Regarding the amount of open dentinal tubules, the highest amount of obstructed dentinal tubules was demonstrated in SnG and nHSnG (p < 0.05) when compared to the others. Between SnG and nHSnG there was no significant difference (p = 0.952) in the amount of closed dentinal tubules in the dentin. Experimental varnishes containing 5% SnCl2 associated or not with 20% nHAP showed to be a promising strategy in preventing erosive-abrasive wear of dentin. In addition, nHSnG was able to obliterate dentinal tubules.
本体外研究评估了一种含有 20%纳米羟基磷灰石（nHAP）和 5%氯化亚锡（SnCl2）的实验性涂料对牛牙本质腐蚀性磨蚀磨损的影响。牛牙颈本质样本先经侵蚀（0.3%柠檬酸，pH2.6，10 分钟），然后随机分为 4 组（n=10）：对照组-不含活性成分的实验性涂料（CG）；含 20%nHAP 的实验性涂料（nHG）；含 5%SnCl2（24800ppmSn2+）的实验性涂料（SnG）；含 20%nHAP 和 5%SnCl2（18300ppmSn2+）的实验性涂料（nHSnG）。涂覆材料后，进行了为期五天的腐蚀性磨蚀挑战。通过三维共焦激光显微镜对侵蚀性牙本质损失和牙本质封闭模式进行分析。采用单向方差分析/ Bonferroni 检验对数据进行分析（α=0.05）。与其他组相比，SnG 和 nHSnG 实验组在预防腐蚀性磨损方面更有效（p<0.05）。SnG 和 nHSnG 组之间的牙本质结构牙本质损失没有统计学差异（p=0.731）。在开放牙本质小管数量方面，与其他组相比，SnG 和 nHSnG 组显示出最多的封闭牙本质小管（p<0.05）。SnG 和 nHSnG 组之间的牙本质中封闭牙本质小管数量没有显著差异（p=0.952）。含 5%SnCl2 的实验性涂料与不含或含有 20%nHAP 的实验性涂料在预防牙本质腐蚀性磨蚀磨损方面显示出有前景的策略。此外，nHSnG 能够封闭牙本质小管。
