J Toxicol Sci. 2009 Oct;34(5):555-62. doi: 10.2131/jts.34.555.
The safety of highly purified fullerenes (HPFs) for utilization as antioxidants in the cosmetic industry was evaluated by studying the toxicity and effects on laboratory animals, human epidermal keratinocytes, and human fibroblasts. The HPFs did not induce primary or cumulative skin irritation, skin sensitization, skin photosensitization or contact phototoxicity. No skin reaction was observed in the patch test on human skin. In the primary eye-irritation test on rabbits, conjunctival redness and corneal epithelial defects were observed in all animals of the eye-unwashed group at 1 and 24 hr after application, but disappeared by 48 hr after application. The irritation may have been caused by administration of insoluble fullerene powder. Therefore, the HPFs were assessed as "minimally irritating" in the eye-irritation test. By comparing these results with previously published data, we concluded that HPFs can be safely used in cosmetic ingredients for human skin application. This is the first study performing all the toxicity tests on the same fullerene material for approval as an additive in quasi-drugs.
我们评估了高纯度富勒烯（HPFs）作为抗氧化剂在化妆品工业中应用的安全性，为此研究了其对实验动物、人体表皮角质细胞和人体成纤维细胞的毒性和影响。HPFs 不会引起原发性或累积性皮肤刺激、皮肤致敏、皮肤光致敏或接触光毒性。在人体皮肤斑贴试验中未观察到皮肤反应。在兔眼刺激性试验中，在施用后 1 小时和 24 小时，所有未经眼部清洗的动物均出现结膜发红和角膜上皮缺损，但在施用后 48 小时后消失。这种刺激可能是由于不溶性富勒烯粉末的给药引起的。因此，在眼刺激性试验中，HPFs 被评估为“轻度刺激性”。通过将这些结果与之前发表的数据进行比较，我们得出结论，HPFs 可以安全地用于人类皮肤应用的化妆品成分。这是第一项对作为准药物添加剂的同种富勒烯材料进行所有毒性试验的研究。
