Son Hyeong-U, Yoon Eun-Kyung, Cha Yong-Soo, Kim Min-A, Shin Yong-Kyu, Kim Jong-Myung, Choi Yong-Hee, Lee Sang-Han
Department of Food Science and Biotechnology, Graduate School, Kyungpook National University, Daegu 702-701;
Exp Ther Med. 2012 Nov;4(5):820-824. doi: 10.3892/etm.2012.677. Epub 2012 Aug 20.
To determine whether aqueous and ethanol fractions of the Angelica keiskei leaf exert toxicity when used for cosmetic purposes, we performed the acute eye irritancy test. Animals were treated with sample fractions (100 mg/dose) according to standard procedure guidelines. No significant changes or damage was detected in the fraction-treated groups in terms of ocular lesions in the cornea, the size of the cornea with turbidity, swelling of the eyelid and emission discharge. However, sodium dioctyl sulfosuccinate, a positive control, induced severe toxic symptoms. Thus, aqueous and ethanol fractions of Angelica keiskei do not appear to induce acute toxicity in the eye lens, as assessed from anatomical and pathological observations in the rabbit eye. Our results collectively suggest that aqueous and ethanol fractions show promise as cosmetic ingredients that do not cause eye toxicity.
为了确定明日叶叶片的水相和乙醇提取物用于化妆品用途时是否具有毒性，我们进行了急性眼刺激试验。按照标准程序指南，用样品提取物（100mg/剂量）处理动物。在角膜眼部病变、角膜浑浊大小、眼睑肿胀和分泌物排放方面，提取物处理组未检测到明显变化或损伤。然而，阳性对照二辛基磺基琥珀酸钠诱导了严重的毒性症状。因此，根据兔眼的解剖学和病理学观察，明日叶的水相和乙醇提取物似乎不会在晶状体中诱发急性毒性。我们的结果共同表明，水相和乙醇提取物有望作为不会引起眼毒性的化妆品成分。
