Usui Tatsufumi, Yamaguchi Tsuyoshi, Ito Hiroshi, Ozaki Hiroichi, Murase Toshiyuki, Ito Toshihiro
The Avian Zoonosis Research Center, Faculty of Agriculture, Tottori University, Koyama, Tottori, Japan.
Virus Genes. 2009 Dec;39(3):319-23. doi: 10.1007/s11262-009-0403-9. Epub 2009 Oct 2.
In April and May 2008, highly pathogenic avian influenza viruses subtype H5N1 were isolated from dead or moribund whooper swans in Aomori, Akita and Hokkaido prefectures in northern Japan. To trace the genetic lineage of the isolates, the nucleotide sequences of all eight genes were determined and phylogenetically analyzed. The Japanese strains were nearly identical to chicken viruses isolated in Russia in April 2008 and closely related to viruses isolated from dead wild birds in Hong Kong in 2007-2008. Their HA genes clustered in clade 2.3.2. On the other hand, NA and the other internal genes were closely related to those of clade 2.3.4 viruses (genotype V) whose NP genes originated from an HA clade 2.3.2 virus. In conclusion, the H5N1 viruses isolated in Japan, Russia and Hong Kong were derived from a common ancestor virus belonging to genotype V that was generated from genetic reassortment events between viruses of HA clades 2.3.2 and 2.3.4.
2008年4月和5月，从日本北部青森、秋田和北海道的死亡或濒死的大天鹅中分离出高致病性H5N1亚型禽流感病毒。为追溯分离株的遗传谱系，测定了所有8个基因的核苷酸序列并进行了系统发育分析。日本的毒株与2008年4月在俄罗斯分离的鸡病毒几乎相同，并且与2007 - 2008年在香港从死亡野生鸟类中分离的病毒密切相关。它们的血凝素（HA）基因聚集在2.3.2分支中。另一方面，神经氨酸酶（NA）和其他内部基因与2.3.4分支病毒（基因型V）的基因密切相关，其核蛋白（NP）基因源自HA 2.3.2分支病毒。总之，在日本、俄罗斯和香港分离出的H5N1病毒源自一个共同的祖先病毒，该病毒属于基因型V，是由HA 2.3.2和2.3.4分支病毒之间的基因重配事件产生的。
