Brumm C, Rivière A, Wilckens C, Löning T
Institute of Pathology, University of Hamburg, Federal Republic of Germany.
Virchows Arch A Pathol Anat Histopathol. 1990;417(6):477-84. doi: 10.1007/BF01625727.
Seventy specimens of normal endometrium (n = 13) and cervix (n = 12), endometrial hyperplasia (n = 4), cervical dysplasia (n = 20), endometrial (n = 11) and cervical carcinoma (n = 8) and uterine metastases of mammary carcinomas (n = 2) have been analysed for c-erB-2 expression with immunohistochemistry employing a monoclonal anti ERBB-2 antibody and Northern-blot hybridization using single stranded RNA probes. In comparison with the c-erbB-2 mRNA expression level found in normal samples, two advanced and poorly differentiated endometrial adenocarcinomas (FIGO IV) and two ductal mammary carcinomas which had metastasized to the uterus, together with three carcinomas in situ of the cervix, showed c-erbB-2 enhanced transcription level. All other endometrial samples including adenomatous hyperplasia and nine endometrial carcinomas (FIGO I), and all other lesions of squamous epithelial origin displayed transcriptional activities at or below the baseline level. Immunohistochemical study of ERBB-2 protein expression showed staining in most samples, although different in distribution and intensity. Staining of endometrial glands was seen in unevenly distributed cells or cell clusters. In contrast, for endocervical glands, labelling was observed distinctly on basally located cells (reserve cells) and at the subapical side of luminal cells. Faint labelling of the basal cell layer was also observed in squamous epithelia. It was more pronounced in severe cervical dysplasia and carcinoma in situ. In carcinomas of glandular origin, dedifferentiation was accompanied by an increase in cytoplasmic labelling, whereas the intensity of staining was not related to differentiation in squamous cell carcinomas. While data derived from Northern blots are suggestive of c-erbB-2 overexpression to indicate an advanced and dedifferentiated state of tumours of glandular origin, staining with an anti-ERBB-2 antibody occurred in both normal and atypical squamous and glandular epithelia and may indicate regular proliferation and/or differentiation-associated events.
采用单克隆抗ERBB - 2抗体通过免疫组织化学法以及使用单链RNA探针的Northern印迹杂交法，对70份正常子宫内膜（n = 13）和宫颈组织（n = 12）、子宫内膜增生（n = 4）、宫颈发育异常（n = 20）、子宫内膜癌（n = 11）、宫颈癌（n = 8）以及乳腺癌子宫转移灶（n = 2）进行了c - erB - 2表达分析。与正常样本中发现的c - erbB - 2 mRNA表达水平相比，2例晚期且低分化的子宫内膜腺癌（国际妇产科联盟IV期）、2例已转移至子宫的乳腺导管癌以及3例宫颈原位癌显示出c - erbB - 2转录水平增强。所有其他子宫内膜样本，包括腺瘤样增生和9例子宫内膜癌（国际妇产科联盟I期），以及所有其他鳞状上皮来源的病变，其转录活性均处于或低于基线水平。ERBB - 2蛋白表达的免疫组织化学研究显示，大多数样本均有染色，尽管分布和强度有所不同。子宫内膜腺体的染色见于分布不均的细胞或细胞簇中。相比之下，对于宫颈内膜腺体，在位于基底的细胞（储备细胞）以及管腔细胞的顶端下侧明显观察到标记。在鳞状上皮中也观察到基底细胞层有微弱标记。在重度宫颈发育异常和原位癌中更为明显。在腺源性癌中，去分化伴随着细胞质标记增加，而鳞状细胞癌中的染色强度与分化无关。虽然Northern印迹数据提示c - erbB - 2过表达表明腺源性肿瘤处于晚期和去分化状态，但抗ERBB - 2抗体染色在正常和非典型鳞状及腺上皮中均有出现，可能表明是正常增殖和/或与分化相关的事件。
