Tsan M F
Blood. 1977 Nov;50(5):935-45.
Phagocytosis and oxidative metabolism of human polymorphonuclear leukocytes (PMN) and rabbit alveolar macrophages (AM) were studied. Human PMN ingested a mean of 12 polyvinyl toluene latex particles (2 micrometer in diameter) per cell. There was stimulation of O2- and H2O2 production, light emission, and activation of the hexose monophosphate shunt during phagocytosis by human PMN. Rabbit AM ingested 51 latex particles (2 micrometer in diameter) per cell. There was no stimulation of the production of O2- and H2O2 or light emission associated with phagocytosis by rabbit AM, while the hexose monophosphate shunt was activated. Similar metabolic changes were obtained in both cell types when opsonized zymosan was used as phagocytic particles. 1-14C-glucoseoxidation was stimulated by H2O2 and methylene blue in both resting human PMN and rabbit AM. It is concluded that activation of the hexose monophosphate shunt in rabbit AM during phagocytosis is independent of O2- and H2O2 production.
对人类多形核白细胞（PMN）和兔肺泡巨噬细胞（AM）的吞噬作用和氧化代谢进行了研究。人类PMN每个细胞平均摄取12个直径为2微米的聚乙烯甲苯乳胶颗粒。在人类PMN吞噬过程中，O2-和H2O2的产生、发光以及磷酸己糖旁路的激活均受到刺激。兔AM每个细胞摄取51个直径为2微米的乳胶颗粒。兔AM吞噬时，未观察到与吞噬作用相关的O2-和H2O2产生或发光的刺激，而磷酸己糖旁路被激活。当用调理酵母聚糖作为吞噬颗粒时，两种细胞类型均出现类似的代谢变化。在静息的人类PMN和兔AM中，H2O2和亚甲蓝均刺激1-14C-葡萄糖氧化。得出的结论是，兔AM在吞噬过程中磷酸己糖旁路的激活与O2-和H2O2的产生无关。
