Ganner Athina, Lienkamp Soeren, Schäfer Tobias, Romaker Daniel, Wegierski Tomasz, Park Tae Joo, Spreitzer Stefan, Simons Matias, Gloy Joachim, Kim Emily, Wallingford John B, Walz Gerd
Renal Division, Department of Medicine, University Hospital Freiburg, Hugstetter Strasse 55, 79106 Freiburg, Germany.
Proc Natl Acad Sci U S A. 2009 Oct 20;106(42):17799-804. doi: 10.1073/pnas.0909465106. Epub 2009 Oct 5.
Planar cell polarity signaling controls a variety of polarized cell behaviors. In multiciliated Xenopus epidermal cells, recruitment of Dishevelled (Dvl) to the basal body and its localization to the center of the ciliary rootlet are required to correctly position the motile cilia. We now report that the anaphase-promoting complex (APC/C) recognizes a D-box motif of Dvl and ubiquitylates Dvl on a highly conserved lysine residue. Inhibition of APC/C function by knockdown of the ANAPC2 subunit disrupts the polarity of motile cilia and alters the directionality of the fluid movement along the epidermis of the Xenopus embryo. Our results suggest that the APC/C activity enables cilia to correctly polarize in Xenopus epidermal cells.
平面细胞极性信号传导控制着多种极化细胞行为。在多纤毛的非洲爪蟾表皮细胞中，将Dishevelled（Dvl）募集到基体并使其定位于纤毛小根的中心是正确定位运动性纤毛所必需的。我们现在报告，后期促进复合物（APC/C）识别Dvl的D框基序并在一个高度保守的赖氨酸残基上使Dvl泛素化。通过敲低ANAPC2亚基来抑制APC/C功能会破坏运动性纤毛的极性，并改变沿非洲爪蟾胚胎表皮的液体流动方向。我们的结果表明，APC/C活性使非洲爪蟾表皮细胞中的纤毛能够正确极化。
