Zweygarth E, Kaminsky R, Webster P
Kenya Trypanosomiasis Research Institute, Kikuyu.
Acta Trop. 1990 Dec;48(2):95-9. doi: 10.1016/0001-706x(90)90048-5.
Bloodstream forms of a stock of Trypanosoma brucei evansi were propagated in vitro for more than 14 months. After that period, all organisms were dyskinetoplastic and had lost their infectivity for mice. An increase in diminazene aceturate resistance in vitro was observed whereas the susceptibility to isometamidium chloride, quinapyramine sulphate and suramin was unaltered. Trypanosomes derived from the long term culture had a surface coat.
伊氏锥虫原种的血流形式在体外传代培养超过14个月。在那段时间之后，所有生物体均为动基体发育不全型，并且对小鼠失去了感染力。观察到体外对乙酰甲喹的耐药性增加，而对氯化异喹胺、硫酸喹啉脲和苏拉明的敏感性未改变。源自长期培养的锥虫具有表面被膜。
