Getova D, Georgiev V, Ivanova V
Experimental Pharmacology Department, Institute of Physiology, Bulgarian Academy of Sciences.
Acta Physiol Pharmacol Bulg. 1990;16(2):42-7.
Comparative tests were performed on male albino rats to study the activity of the original hydroxylamine barbiturate HB-7, with phenobarbital (PhB) and pentobarbital (PB) using the conflict situation test (after Vogel et al., 1971) with thirsty rats (deprived of water for 48 hours). The three barbiturates studied (HB-7, PhB and PB) were administered intraperitoneally in four doses (5, 10, 15 and 20 mg/kg). Two parameters were registered: number of punished responses (electrical shocks punishing attempts to drink) and the number of non-punished responses (approaching the drinking tube) for the 5-min period of observation. In a second group of experiments the same test was used to study the effect of picrotoxin and of its combinations with the effective doses of HB-7, PhB and PB. The three barbiturates tested were found to have an anxiolytic effect, i.e. to increase the number of attempts to drink water, in the higher doses used. Picrotoxin causes a decrease in the number of punished responses (anxiomimetic effect). When HB-7, PhB and PB are combined with picrotoxin, the latter eliminates their anxiolytic action. The conclusion reached is that the anxiolytic action of the barbiturates tested is probably connected with an effect on the GABA-ergic transmitter system in the central nervous system.
对雄性白化大鼠进行了对比试验，以研究原羟基胺巴比妥酸盐HB - 7与苯巴比妥（PhB）和戊巴比妥（PB）的活性，采用冲突情境试验（根据Vogel等人，1971年），使用口渴的大鼠（剥夺水分48小时）。所研究的三种巴比妥酸盐（HB - 7、PhB和PB）以四种剂量（5、10、15和20毫克/千克）腹腔注射给药。记录了两个参数：在5分钟观察期内受罚反应的次数（电击惩罚饮水尝试）和未受罚反应的次数（接近饮水管）。在第二组实验中，使用相同的试验来研究印防己毒素及其与有效剂量的HB - 7、PhB和PB组合的效果。所测试的三种巴比妥酸盐在使用较高剂量时被发现具有抗焦虑作用，即增加饮水尝试的次数。印防己毒素导致受罚反应次数减少（拟焦虑作用）。当HB - 7、PhB和PB与印防己毒素联合使用时，后者消除了它们的抗焦虑作用。得出的结论是，所测试的巴比妥酸盐的抗焦虑作用可能与对中枢神经系统中γ-氨基丁酸能递质系统的影响有关。
