College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
Heilongjiang Key Laboratory of Animals Disease Pathogenesis and Comparative Medicine, Harbin, China.
FEBS Open Bio. 2023 Aug;13(8):1485-1494. doi: 10.1002/2211-5463.13613. Epub 2023 Jun 11.
Osteoarthritis (OA) is a chronic degenerative bone and joint disease that often occurs in aging animals. Currently, there are still no biomarkers that can effectively diagnose OA in the early stage. To identify possible biomarkers, here we examined changes in the expression of C-telopeptide fragments of type II collagen (CTX-II) and collagenase generated carboxy-terminal neoepitope of type II collagen (C2C) in serum at different time points in an anterior cruciate ligament transection (ACLT)-induced rat OA model. The serum levels of CTX-II and C2C, and the OARSI score in the ACLT group were increased from week two until the end of the experiment. The AUC of the combined biomarkers was higher than that of CTX-II or C2C alone. Moreover, serum levels of CTX-II and C2C were positively correlated with the OARSI score. The results suggest that the combined detection of serum CTX-II and C2C concentrations may have potential for assessing and diagnosing OA at early stages.
骨关节炎（OA）是一种常见于老年动物的慢性退行性骨和关节疾病。目前，仍然没有可以有效诊断 OA 早期的生物标志物。为了确定可能的生物标志物，我们在这里检查了在前交叉韧带切断（ACLT）诱导的大鼠 OA 模型中不同时间点血清中 II 型胶原 C 端肽片段（CTX-II）和 II 型胶原产生的胶原酶羧基末端新表位（C2C）的表达变化。ACLT 组的 CTX-II 和 C2C 血清水平以及 OARSI 评分从第 2 周开始增加，直到实验结束。联合生物标志物的 AUC 高于 CTX-II 或 C2C 单独的 AUC。此外，CTX-II 和 C2C 的血清水平与 OARSI 评分呈正相关。结果表明，联合检测血清 CTX-II 和 C2C 浓度可能具有评估和诊断 OA 早期的潜力。
