Institute of Pathology, 30625, Hannover, Germany.
Platelets. 2009 Sep;20(6):391-400. doi: 10.1080/09537100903114537.
In primary myelofibrosis (PMF) and essential thrombocythemia (ET) the megakaryocytic lineage characteristically shows aberrant proliferation and maturation in which the regulatory microRNA (miR) system might be involved. Laser-microdissected PMF and ET megakaryocytes were analysed with real-time polymerase chain reaction (PCR) low density arrays comprising 365 microRNAs. The highest megakaryocytic expression levels were observed for miR-223, which is known to be expressed also in granulopoiesis. Cluster analysis revealed a tendency of disease-specific megakaryocytic microRNA expression profiles indicating that a complex shift of microRNA expression appears to be the underlying defect. Increased accumulation of miR-146b was observed in cellular stage PMF (p = 0.0125) but not ET megakaryopoiesis. In conclusion, this is the first microRNA profiling of in situ-derived PMF, ET and normal megakaryocytes.
在原发性骨髓纤维化（PMF）和特发性血小板增多症（ET）中，巨核细胞谱系表现出异常增殖和成熟，其中调节 microRNA（miR）系统可能参与其中。使用包含 365 种 microRNAs 的实时聚合酶链反应（PCR）低密度阵列分析了激光微切割的 PMF 和 ET 巨核细胞。miR-223 的巨核细胞表达水平最高，已知其在粒细胞生成中也有表达。聚类分析显示出疾病特异性巨核细胞 microRNA 表达谱的趋势，表明 microRNA 表达的复杂转移似乎是潜在的缺陷。在细胞阶段 PMF 中观察到 miR-146b 的积累增加（p=0.0125），但 ET 巨核细胞生成中没有增加。总之，这是对原位衍生的 PMF、ET 和正常巨核细胞进行的首次 microRNA 分析。
