通过用不同摩尔浓度的氯化钠处理，从拉吉细胞和染色质中溶解爱泼斯坦-巴尔病毒（EBV）相关核抗原。
Solubilisation of Epstein-Barr virus (EBV)-associated nuclear antigen from Raji cells and chromatin by treatment with various molarities of NaCl.
作者信息
Brown T D, Rickwood D, MacGillivray A J, Klein G
出版信息
Cancer Lett. 1977 Sep;3(3-4):151-6. doi: 10.1016/s0304-3835(77)95187-4.
The solubilisation of Epstein-Barr virus (EBV)-associated nuclear antigen (EBNA) by treatment with various molarities of NaCl was investigated using the 125I--IgG absorption assay. Ninety percent of the antigenic activity detected using the 125I--IgG absorption assay was insoluble at 0.15 M NaCl. It could be rendered soluble by treatment with 2.0 M NaCl, but reprecipitated upon return to 0.15 M NaCl. EBNA was partially extracted from Raji chromatin by treatment with 0.35 M NaCl. The efficiency of extraction was increased by homogenisation in 2.0 M NaCl followed by dialysis to 0.35 M NaCl. The data demonstrate the close association of EBNA with Raji chromatin and suggest that it may be a chromatin-associated non-histone protein.
采用¹²⁵I-IgG吸收试验，研究了用不同摩尔浓度的NaCl处理对爱泼斯坦-巴尔病毒（EBV）相关核抗原（EBNA）溶解性的影响。用¹²⁵I-IgG吸收试验检测到的90%的抗原活性在0.15M NaCl中不溶。用2.0M NaCl处理可使其溶解，但回到0.15M NaCl时又会重新沉淀。用0.35M NaCl处理可从拉吉细胞染色质中部分提取出EBNA。在2.0M NaCl中匀浆后透析至0.35M NaCl可提高提取效率。数据表明EBNA与拉吉细胞染色质紧密相关，并提示它可能是一种与染色质相关的非组蛋白。
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