爱泼斯坦-巴尔病毒抗原的特性。I. 补体结合可溶性抗原的生化分析及其与爱泼斯坦-巴尔病毒相关核抗原的关系。
Characterization of Epstein-Barr virus antigens. I. Biochemical analysis of the complement-fixing soluble antigen and relationship with Epstein-Barr virus-associated nuclear antigen.
作者信息
Lenoir G, Berthelon M C, Favre M C, de-Thé G
出版信息
J Virol. 1976 Feb;17(2):672-4. doi: 10.1128/JVI.17.2.672-674.1976.
Abstract
The Epstein-Barr virus-soluble (S) antigen extracted from RAJI cells was characterized by sucrose gradient centrifugation, gel filtration, and ion-exchange chromatography. The sedimentation coefficient was estimated to be 8.5S corresponding to a molecular weight of 180,000. The S antigen binds to DEAE-A25 ion exchanger from which it can be eluted with 0.3 M NaCl in Tris buffer (pH 7.2). All fractions which contained complement-fixing S antigen also inhibited the anticomplement immunofluorescence reaction as used to detect the Epstein-Barr virus-associated nuclear antigen. These results are consistent with the hypothesis that the S and Epstein-Barr virus-associated nuclear antigens are either a single antigen or that both activities are present on the same molecule.
摘要
从RAJI细胞中提取的爱泼斯坦-巴尔病毒可溶性（S）抗原，通过蔗糖梯度离心、凝胶过滤和离子交换色谱进行了特性分析。沉降系数估计为8.5S，对应分子量为180,000。S抗原与DEAE-A25离子交换剂结合，可用Tris缓冲液（pH 7.2）中的0.3M NaCl将其洗脱。所有含有补体结合性S抗原的组分也抑制了用于检测爱泼斯坦-巴尔病毒相关核抗原的抗补体免疫荧光反应。这些结果与以下假设一致，即S抗原和爱泼斯坦-巴尔病毒相关核抗原要么是单一抗原，要么两种活性存在于同一分子上。
相似文献
1
Characterization of Epstein-Barr virus antigens. I. Biochemical analysis of the complement-fixing soluble antigen and relationship with Epstein-Barr virus-associated nuclear antigen.爱泼斯坦-巴尔病毒抗原的特性。I. 补体结合可溶性抗原的生化分析及其与爱泼斯坦-巴尔病毒相关核抗原的关系。
J Virol. 1976 Feb;17(2):672-4. doi: 10.1128/JVI.17.2.672-674.1976.
2
Partial purification of the Epstein-Barr virus nuclear antigen(s).爱泼斯坦-巴尔病毒核抗原的部分纯化
J Biol Chem. 1983 Mar 25;258(6):3974-82.
3
Purification and biochemical characterization of the Epstein-Barr virus-determined nuclear antigen and an associated protein with a 53,000-dalton subunit.爱泼斯坦-巴尔病毒决定的核抗原及一种具有53,000道尔顿亚基的相关蛋白的纯化与生化特性分析
J Virol. 1980 Sep;35(3):592-602. doi: 10.1128/JVI.35.3.592-602.1980.
4
Purification of the Epstein-Barr virus-determined nuclear antigen from Epstein-Barr virus-transformed human lymphoid cell lines.从爱泼斯坦-巴尔病毒转化的人淋巴母细胞系中纯化爱泼斯坦-巴尔病毒决定的核抗原。
J Virol. 1978 Sep;27(3):604-11. doi: 10.1128/JVI.27.3.604-611.1978.
5
6
Study of Epstein-Barr virus-determined nuclear antigen (EBNA) by chromatography on fixed cell nuclei.通过固定细胞核上的色谱法对爱泼斯坦-巴尔病毒确定的核抗原（EBNA）进行研究。
Int J Cancer. 1978 Nov 15;22(5):535-41. doi: 10.1002/ijc.2910220505.
7
Identification of a purified complement-fixing antigen as the Epstein-Barr-virus determined nuclear antigen (EBNA) by its binding to metaphase chromosomes.
Proc Natl Acad Sci U S A. 1977 Apr;74(4):1605-9. doi: 10.1073/pnas.74.4.1605.
8
Soluble complement fixing antigens in Epstein-Barr virus cell systems.爱泼斯坦-巴尔病毒细胞系统中的可溶性补体结合抗原。
Bibl Haematol. 1975 Oct(43):412-6. doi: 10.1159/000399181.
9
Solubilisation of Epstein-Barr virus (EBV)-associated nuclear antigen from Raji cells and chromatin by treatment with various molarities of NaCl.通过用不同摩尔浓度的氯化钠处理，从拉吉细胞和染色质中溶解爱泼斯坦-巴尔病毒（EBV）相关核抗原。
Cancer Lett. 1977 Sep;3(3-4):151-6. doi: 10.1016/s0304-3835(77)95187-4.
10
Identification of an Epstein-Barr virus nuclear antigen by fluoroimmunoelectrophoresis and radioimmunoelectrophoresis.通过荧光免疫电泳和放射免疫电泳鉴定爱泼斯坦-巴尔病毒核抗原。
J Virol. 1981 Jun;38(3):996-1004. doi: 10.1128/JVI.38.3.996-1004.1981.
引用本文的文献
1
No activation of new initiation points for deoxyribonucleic acid replication in BALB/c 3T3 cells transformed by Kirsten sarcoma virus.在由 Kirsten 肉瘤病毒转化的 BALB/c 3T3 细胞中，未激活用于脱氧核糖核酸复制的新起始点。
Mol Cell Biol. 1981 Aug;1(8):763-8. doi: 10.1128/mcb.1.8.763-768.1981.
2
Initiation points for cellular deoxyribonucleic acid replication in human lymphoid cells converted by Epstein-Barr virus.由爱泼斯坦 - 巴尔病毒转化的人类淋巴细胞中细胞脱氧核糖核酸复制的起始点
Mol Cell Biol. 1981 Aug;1(8):753-62. doi: 10.1128/mcb.1.8.753-762.1981.
3
Antibodies to the Epstein-Barr virus nuclear antigen and to rheumatoid arthritis nuclear antigen identify the same polypeptide.针对爱泼斯坦-巴尔病毒核抗原和类风湿性关节炎核抗原的抗体识别出同一种多肽。
Proc Natl Acad Sci U S A. 1983 Dec;80(23):7104-8. doi: 10.1073/pnas.80.23.7104.
4
One of two Epstein-Barr virus nuclear antigens contains a glycine-alanine copolymer domain.两种爱泼斯坦-巴尔病毒核抗原之一含有一个甘氨酸-丙氨酸共聚结构域。
Proc Natl Acad Sci U S A. 1983 Sep;80(18):5665-9. doi: 10.1073/pnas.80.18.5665.
5
Induction of Epstein-Barr virus nuclear antigen and DNA synthesis in a human epithelial cell line after Epstein-Barr virus infection.爱泼斯坦-巴尔病毒感染后人类上皮细胞系中爱泼斯坦-巴尔病毒核抗原的诱导及DNA合成
J Virol. 1982 Feb;41(2):703-8. doi: 10.1128/JVI.41.2.703-708.1982.
6
Evidence for two classes of chromatin-associated Epstein-Barr virus-determined nuclear antigen.两类与染色质相关的爱泼斯坦-巴尔病毒确定的核抗原的证据。
J Virol. 1982 Aug;43(2):555-65. doi: 10.1128/JVI.43.2.555-565.1982.
7
Purification and biochemical characterization of the Epstein-Barr virus-determined nuclear antigen and an associated protein with a 53,000-dalton subunit.爱泼斯坦-巴尔病毒决定的核抗原及一种具有53,000道尔顿亚基的相关蛋白的纯化与生化特性分析
J Virol. 1980 Sep;35(3):592-602. doi: 10.1128/JVI.35.3.592-602.1980.
8
Purification of the Epstein-Barr virus-determined nuclear antigen from Epstein-Barr virus-transformed human lymphoid cell lines.从爱泼斯坦-巴尔病毒转化的人淋巴母细胞系中纯化爱泼斯坦-巴尔病毒决定的核抗原。
J Virol. 1978 Sep;27(3):604-11. doi: 10.1128/JVI.27.3.604-611.1978.
9
Epstein-barr virus-specific RNA. II. Analysis of polyadenylated viral RNA in restringent, abortive, and prooductive infections.爱泼斯坦-巴尔病毒特异性RNA。II. 对受限、流产和 productive 感染中多聚腺苷酸化病毒RNA的分析。
注：这里“productive”原词有误，应该是“productive”，正确翻译为“增殖性” ，整句译文为：爱泼斯坦-巴尔病毒特异性RNA。II. 对受限、流产和增殖性感染中多聚腺苷酸化病毒RNA的分析。
J Virol. 1977 May;22(2):321-30. doi: 10.1128/JVI.22.2.321-330.1977.
10
Solubilization of the Epstein-Barr virus-determined nuclear antigen and its characterization as a DNA-binding protein.爱泼斯坦-巴尔病毒决定的核抗原的溶解及其作为一种DNA结合蛋白的特性鉴定。
J Virol. 1977 Apr;22(1):1-8. doi: 10.1128/JVI.22.1.1-8.1977.
本文引用的文献
1
Application of a microtechnique to viral serological investigations.一种微技术在病毒血清学研究中的应用。
J Immunol. 1962 Mar;88:320-9.
2
Epstein-Barr virus-induced viral and soluble complement-fixing antigens in Burkitt lymphoma cell cultures.伯基特淋巴瘤细胞培养物中爱泼斯坦-巴尔病毒诱导的病毒及可溶性补体结合抗原
Proc Soc Exp Biol Med. 1970 Jul;134(3):748-51. doi: 10.3181/00379727-134-34875.
3
4
Isolation and characterization of a large molecular-weight polypeptide of herpes simplex virus type 1.1型单纯疱疹病毒一种大分子多肽的分离与鉴定
Virology. 1974 Dec;62(2):539-51. doi: 10.1016/0042-6822(74)90414-0.
5
Proteins specified by herpes simplex virus. XI. Identification and relative molar rates of synthesis of structural and nonstructural herpes virus polypeptides in the infected cell.单纯疱疹病毒指定的蛋白质。十一。感染细胞中单纯疱疹病毒结构和非结构多肽的合成鉴定及相对摩尔率。
J Virol. 1973 Dec;12(6):1347-65. doi: 10.1128/JVI.12.6.1347-1365.1973.
6
Significance of a complement-fixing antigen associated with herpes-like virus and detected in the Raji cell line.与疱疹样病毒相关且在Raji细胞系中检测到的补体结合抗原的意义。
Nature. 1969 Apr 12;222(5189):186-7. doi: 10.1038/222186a0.
7
Epstein-Barr virus-associated complement-fixing and nuclear antigens in Burkitt lymphoma biopsies.伯基特淋巴瘤活检组织中与爱泼斯坦-巴尔病毒相关的补体结合抗原和核抗原
Int J Cancer. 1974 Jun 15;13(6):755-63. doi: 10.1002/ijc.2910130604.
8
Cellular localization of an Epstein-Barr virus (EBV)-associated complement-fixing antigen in producer and non-producer lymphoblastoid cell lines.爱泼斯坦-巴尔病毒（EBV）相关补体结合抗原在产生病毒和不产生病毒的淋巴母细胞系中的细胞定位。
Int J Cancer. 1973 May;11(3):499-520. doi: 10.1002/ijc.2910110302.
9
Nasopharyngeal carcinoma. IX. Antibodies to EBNA and correlation with response to other ebv antigens in chinese patients.鼻咽癌。IX. 中国患者中针对EBNA的抗体及其与对其他EBV抗原反应的相关性
Int J Cancer. 1975 Nov 15;16(5):713-21. doi: 10.1002/ijc.2910160503.
10
Lymphoblastoid transformation and kinetics of appearance of viral nuclear antigen (EBNA) in cord-blood lymphocytes infected by Epstein-Barr Virus (EBV).爱泼斯坦-巴尔病毒（EBV）感染的脐血淋巴细胞中的淋巴母细胞转化及病毒核抗原（EBNA）出现的动力学
Int J Cancer. 1975 Mar 15;15(3):377-84. doi: 10.1002/ijc.2910150303.
Zotero 插件