Martinez A, Pailhoux E, Berger M, Jean C
Physiologie Comparée et Endocrinologie, CNRS UA 360, Université Blaise Pascal, Aubiere, France.
Mol Cell Endocrinol. 1990 Sep 10;72(3):201-11. doi: 10.1016/0303-7207(90)90144-w.
A cDNA encoding the major mouse vas deferens protein (MVDP) has been cloned and characterized. Using in situ hybridization we have identified the epithelial cells of the vas deferens as the site of synthesis of MVDP mRNA. Northern blot analysis suggests that a high level of an mRNA corresponding to the MVDP gene is present in the mouse vas deferens whereas the amount of MVDP mRNA in vas deferens of other species studied, or in other mouse tissues, even if present, is undetectable. Steady-state levels of MVDP mRNA are decreased by approximately 42% 3 days after castration but a significant hybridization signal is still observed even 50 days after castration. Testosterone treatment for 2 weeks is necessary to completely reverse the effect of castration. In vitro transcription assays on isolated nuclei showed that the hormonal induction of the MVDP gene is achieved mainly at transcriptional level.
一种编码主要小鼠输精管蛋白（MVDP）的cDNA已被克隆并进行了表征。通过原位杂交，我们确定输精管的上皮细胞是MVDP mRNA的合成部位。Northern印迹分析表明，与MVDP基因相对应的高水平mRNA存在于小鼠输精管中，而在所研究的其他物种的输精管中，或在其他小鼠组织中，即使存在MVDP mRNA，其含量也无法检测到。去势后3天，MVDP mRNA的稳态水平下降约42%，但即使在去势后50天仍能观察到明显的杂交信号。需要进行2周的睾酮治疗才能完全逆转去势的影响。对分离细胞核进行的体外转录分析表明，MVDP基因的激素诱导主要在转录水平实现。
