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比较两种显色培养基对粪便标本中耐万古霉素肠球菌的选择性分离。

Comparison of two chromogenic media for selective isolation of vancomycin-resistant enterococci from stool specimens.

机构信息

Institute of Medical Microbiology, RWTH Aachen University Hospital, Aachen, Germany.

出版信息

J Clin Microbiol. 2009 Dec;47(12):4113-6. doi: 10.1128/JCM.00882-09. Epub 2009 Oct 7.

DOI:10.1128/JCM.00882-09
PMID:19812271
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2786630/
Abstract

Two chromogenic media (Chromagar VRE and chromID VRE [C-ID]) performed equally well in the direct detection of vancomycin-resistant enterococci (VRE) in stool specimens after an overnight enrichment step and a 48-h incubation period, with a sensitivity of 98.2% (56/57) for both and specificities of 96.5% (195/202) and 97.5% (197/202), respectively. However, assigning discriminatory colony color was sometimes difficult, especially on C-ID. In order to facilitate simple species identification, biochemical key reactions were implemented.

摘要

两种显色培养基（Chromagar VRE 和 chromID VRE [C-ID]）在经过过夜增菌和 48 小时孵育后，对粪便标本中万古霉素耐药肠球菌（VRE）的直接检测效果相当，其灵敏度分别为 98.2%（56/57）和 96.5%（195/202）、97.5%（197/202）。然而，有时区分菌落颜色比较困难，尤其是在 C-ID 上。为了便于简单的种属鉴定，实施了生化关键反应。

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J Clin Microbiol. 2007 Aug;45(8):2731-3. doi: 10.1128/JCM.00448-07. Epub 2007 Jun 6.
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