用生化鉴定法鉴定肠球菌属菌种。
Identification of Enterococcus spp. with a biochemical key.
作者信息
Manero A, Blanch A R
机构信息
Departament de Microbiologia, Universitat de Barcelona, E-08028 Barcelona, Catalonia, Spain.
出版信息
Appl Environ Microbiol. 1999 Oct;65(10):4425-30. doi: 10.1128/AEM.65.10.4425-4430.1999.
Abstract
A six-step biochemical key is presented for the identification of all recognized Enterococcus spp. The key consists of 12 tests, but no more than 6 are needed for the most complicated identification. The reliability of the key has been evaluated with collection type strains and clinical and environmental isolates. This key has fewer tests than those reported in previous studies. There is no commercial kit that includes the whole set of tests. However, some of the tests are included in enzyme activity-based kits that could be used with the proposed key. The key is designed for use in routine applications, especially in environmental and clinical studies with a high number of isolates.
摘要
本文提出了一个用于鉴定所有已知肠球菌属物种的六步生化鉴定法。该鉴定法包含12项检测，但对于最复杂的鉴定，所需检测不超过6项。我们已使用收集的模式菌株、临床和环境分离株对该鉴定法的可靠性进行了评估。此鉴定法的检测项目比以往研究报告的要少。目前没有包含整套检测项目的商业试剂盒。不过，基于酶活性的试剂盒中包含了部分检测项目，可与本文提出的鉴定法配合使用。该鉴定法专为常规应用设计，尤其适用于有大量分离株的环境和临床研究。
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