Comparative Genomics Unit, Institute of Genomics and Integrative Biology, Delhi University Campus, Mall Road, Delhi, 110007, India.
Mol Cell Biochem. 2010 Mar;336(1-2):127-35. doi: 10.1007/s11010-009-0263-6. Epub 2009 Oct 8.
Whole plant methanolic extracts of 14 traditionally used medicinal herbs were evaluated for their anti-inflammatory activity. Extracts of Grindelia robusta, Salix nigra, Arnica montana, and Quassia amara showed up to 4.5-fold inhibition of nitric oxide (NO) production in the J774 murine macrophage cells challenged with LPS without cytotoxicity. These four selected extracts significantly reduced the protein levels of inducible NO synthase (iNOS) and the cyclooxygenase-2 (COX-2) as observed by Western blot analysis. Culture supernatants from cells treated with these extracts indicated 3-5-fold reduction of tumor necrosis factor-alpha (TNF-alpha). However, only G. robusta and Q. amara extracts significantly inhibited (by 50%) IL-1beta and IL-12 secretions. Furthermore, all these plant extracts were shown to prevent the LPS-mediated nuclear translocation of nuclear factor-kappaB (NF-kappaB). All the above observations indicate the anti-inflammatory potential of these plant extracts.
对 14 种传统药用植物的全植物甲醇提取物进行了抗炎活性评价。在 LPS 刺激的 J774 鼠巨噬细胞中，Grindelia robusta、Salix nigra、Arnica montana 和 Quassia amara 的提取物对一氧化氮（NO）的产生具有高达 4.5 倍的抑制作用，且无细胞毒性。这四种选定的提取物通过 Western blot 分析显著降低了诱导型一氧化氮合酶（iNOS）和环氧化酶-2（COX-2）的蛋白水平。用这些提取物处理细胞的培养上清液表明肿瘤坏死因子-α（TNF-α）减少了 3-5 倍。然而，只有 G. robusta 和 Q. amara 提取物能显著抑制（50%）IL-1beta 和 IL-12 的分泌。此外，所有这些植物提取物都能防止 LPS 介导的核因子-kappaB（NF-kappaB）的核转位。所有这些观察结果表明这些植物提取物具有抗炎潜力。
