Key Laboratory for Natural Resource of Changbai Mountain & Functional Molecules, Ministry of Education, College of Pharmacy, Yanbian University, Jilin Province, China.
J Pharm Pharmacol. 2009 Oct;61(10):1375-82. doi: 10.1211/jpp/61.10.0015.
The aim was to investigate the protective effect of salidroside isolated from Rhodiola sachalinensis A. Bor. (Crassulaceae) on D-galactosamine/lipopolysaccharide-induced fulminant hepatic failure.
Hepatotoxicity was induced by an intraperitoneal injection of D-galactosamine (700 mg/kg) and lipopolysaccharide (10 mug/kg); salidroside (20, 50 and 100 mg/kg) was administered intraperitoneally 1 h before induction of hepatoxicity. Liver injury was assessed biochemically and histologically.
Salidroside attenuated the induced acute increase in serum aspartate aminotransferase and alanine aminotransferase activities, and levels of tumour necrosis factor-alpha levels and serum nitric oxide. It restored depleted hepatic glutathione, superoxide dismutase, catalase and glutathione peroxidase activities, decreased malondialdehyde levels and considerably reduced histopathological changes. Histopathological, immunohistochemical and Western blot analyses also demonstrated that salidroside could reduce the appearance of necrotic regions and expression of caspase-3 and hypoxia-inducible factor-1alpha in liver tissue.
Salidroside protected liver tissue from the oxidative stress elicited by D-galactosamine and lipopolysaccharide. The hepatoprotective mechanism of salidroside appear to be related to antioxidant activity and inhibition of hypoxia-inducible factor-1alpha.
研究来自高山红景天（景天科）的红景天苷对 D-半乳糖胺/脂多糖诱导的暴发性肝衰竭的保护作用。
通过腹腔注射 D-半乳糖胺（700mg/kg）和脂多糖（10μg/kg）诱导肝毒性；红景天苷（20、50 和 100mg/kg）在诱导肝毒性前 1 小时腹腔内给药。通过生化和组织学评估肝损伤。
红景天苷减轻了诱导的血清天冬氨酸转氨酶和丙氨酸转氨酶活性、肿瘤坏死因子-α水平和血清一氧化氮水平的急性升高。它恢复了耗竭的肝谷胱甘肽、超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性，降低了丙二醛水平，并显著减少了组织病理学变化。组织病理学、免疫组织化学和 Western blot 分析还表明，红景天苷可减少肝组织中坏死区域和 caspase-3 和缺氧诱导因子-1α的表达。
红景天苷可保护肝组织免受 D-半乳糖胺和脂多糖引起的氧化应激。红景天苷的保肝机制可能与抗氧化活性和抑制缺氧诱导因子-1α有关。
