Pfingstgraf Iulia Olimpia, Taulescu Marian, Pop Raluca Maria, Orăsan Remus, Vlase Laurian, Uifalean Ana, Todea Doina, Alexescu Teodora, Toma Corina, Pârvu Alina Elena
Department of Pathophysiology, Faculty of Medicine, Iuliu Haţieganu University of Medicine and Pharmacy Cluj-Napoca, 400012 Cluj-Napoca, Romania.
Department of Pathology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 400372 Cluj-Napoca, Romania.
Antioxidants (Basel). 2021 Mar 24;10(4):504. doi: 10.3390/antiox10040504.
(TO) or dandelion has been frequently used to prevent or treat different liver diseases because of its rich composition in phytochemicals with demonstrated effect against hepatic injuries. This study aimed to investigate the possible preventing effect of ethanolic TO root extract (TOERE) on a rat experimental acute on chronic liver failure (ACLF) model.
Chronic liver failure (CLF) was induced by human serum albumin, and ACLF was induced in CLF by D-galactosamine and lipopolysaccharide (D-Gal-LPS). Five groups ( = 5) of male Wistar rats (200-250 g) were used: ACLF, ACLF-silymarin (200 mg/kg b.w./day), three ACLF-TO administered in three doses (200 mg, 100 mg, 50 mg/kg b.w./day).
The in vivo results showed that treatment with TOERE administered in three chosen doses before ACLF induction reduced serum liver injury markers (AST, ALT, ALP, GGT, total bilirubin), renal tests (creatinine, urea), and oxidative stress tests (TOS, OSI, MDA, NO, 3NT). Histopathologically, TOERE diminished the level of liver tissue injury and 3NT immunoexpression.
This paper indicated oxidative stress reduction as possible mechanisms for the hepatoprotective effect of TOERE in ACLF and provided evidence for the preventive treatment.
蒲公英（TO）因其富含具有抗肝损伤作用的植物化学物质，常被用于预防或治疗各种肝脏疾病。本研究旨在探讨蒲公英根乙醇提取物（TOERE）对大鼠实验性急性-on-慢性肝衰竭（ACLF）模型的可能预防作用。
用人血清白蛋白诱导慢性肝衰竭（CLF），并用D-半乳糖胺和脂多糖（D-Gal-LPS）在CLF基础上诱导ACLF。使用五组（每组n = 5）雄性Wistar大鼠（200 - 250 g）：ACLF组、ACLF-水飞蓟宾组（200 mg/kg体重/天）、三个ACLF-TO组，分别给予三种剂量（200 mg、100 mg、50 mg/kg体重/天）。
体内实验结果表明，在诱导ACLF前给予三种选定剂量的TOERE治疗可降低血清肝损伤标志物（AST、ALT、ALP、GGT、总胆红素）、肾脏指标（肌酐、尿素）和氧化应激指标（TOS、OSI、MDA、NO、3NT）。组织病理学结果显示，TOERE减轻了肝组织损伤程度和3NT免疫表达。
本文指出氧化应激降低可能是TOERE对ACLF具有肝保护作用的机制，并为预防性治疗提供了证据。
