Troudi Afef, Mahjoubi Samet Amira, Zeghal Najiba
Department of Life Sciences, Animal Physiology Laboratory, Sciences Faculty of Sfax, Sfax, Tunisia.
Exp Toxicol Pathol. 2010 Nov;62(6):637-42. doi: 10.1016/j.etp.2009.08.010. Epub 2009 Oct 7.
Gibberellic acid (GA(3)), a plant growth regulator, was largely used in agriculture of many countries including Tunisia. However, its potential hazardous effects on human health were relatively unexplored. The purpose of this study was to investigate the effects of GA(3) on hepatic function in female rats and their pups. Animals were given daily 200 ppm GA(3) in drinking water from the 14th day of pregnancy until day 14 after delivery. It was found that GA(3) induced liver damages as evidenced by the elevation of plasma aminotransferases (ALT, AST), lactate dehydrogenase activities, bilirubin and albumin levels. Hepatotoxicity was objectified by the significant increase of malondialdehyde (MDA) level and a decrease of antioxidant enzyme activities such as catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione content in liver of suckling pups and their mothers. Impairment of hepatic function corresponded histologically. We have observed blood vessels congestion and leucocytes infiltration, which were more pronounced in hepatocytes of dams than those of suckling pups. Results of this current study suggest that exposure rats to GA(3) induces hepatotoxicity and histopathological changes in liver of female rats and their progeny.
赤霉素（GA(3)）是一种植物生长调节剂，在包括突尼斯在内的许多国家的农业中被大量使用。然而，其对人类健康的潜在危害作用相对未被充分研究。本研究的目的是调查GA(3)对雌性大鼠及其幼崽肝脏功能的影响。从怀孕第14天到产后第14天，给动物每日饮用含200 ppm GA(3)的水。结果发现，GA(3)可导致肝脏损伤，血浆氨基转移酶（ALT、AST）、乳酸脱氢酶活性、胆红素和白蛋白水平升高即为证据。丙二醛（MDA）水平显著升高以及抗氧化酶活性降低，如过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）以及哺乳幼崽及其母亲肝脏中的谷胱甘肽含量降低，这些都证实了肝毒性。肝脏功能损害在组织学上也有相应表现。我们观察到血管充血和白细胞浸润，在母鼠肝细胞中比在哺乳幼崽肝细胞中更为明显。本研究结果表明，大鼠接触GA(3)会导致雌性大鼠及其后代肝脏产生肝毒性和组织病理学变化。
