Khayal Eman El-Sayed, Elhadidy Mona G, Alnasser Sulaiman Mohammed, Morsy Manal Mohammad, Farag Azza I, El-Nagdy Samah A
Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Zagazig University, Egypt.
Department of Medical Physiology, Faculty of Medicine, Mansoura University, Egypt.
Toxicol Rep. 2024 Dec 27;14:101882. doi: 10.1016/j.toxrep.2024.101882. eCollection 2025 Jun.
Extensive uses of silver nanoparticles (Ag NPs) in different industries result in exposure to these nanoparticle imperatives in our daily lives. Resveratrol is found in many plants as a natural compound. The present study aimed to estimate the renal toxic effects of Ag NPs in adult male albino rats and the underlying relevant mechanisms while studying the possible role of resveratrol in ameliorating these effects. Thirty adult albino rats were split into 5 groups; control, vehicle, resveratrol (30 mg/kg), Ag NPs (300 mg/kg), and resveratrol + Ag NPs groups. The treatments were given orally for 4 weeks. Ag NPs group displayed a reduction in kidney weight ( absolute and relative), excess in urinary levels of kidney injury molecule, neutrophil gelatinase-associated lipocalin, cystatin, and blood kidney biomarkers (creatinine, urea, and potassium), increases in oxidative stress markers with the reduction in antioxidant markers, and decreases in serum sirtuin 1(SIRT1) level. Upregulation of interleukin 1 beta, tumor necrosis factor-alpha, and monocyte chemoattractant protein-1 gene expressions with downregulation of nephrin and podocin gene expressions in renal tissues were also observed. These changes were associated with histological alterations of the glomeruli and tubules, and increased area percentage of collagen fiber. A significant increase in the optical density of 1 and claudin-1 immunostaining was detected in the Ag NPs group when compared to other groups. All these changes were alleviated by the usage of resveratrol through its anti-oxidant, anti-inflammatory, and activation of SIRT1 recommending its use as a renoprotective agent.
银纳米颗粒（Ag NPs）在不同行业的广泛应用导致我们在日常生活中不可避免地接触到这些纳米颗粒。白藜芦醇作为一种天然化合物存在于许多植物中。本研究旨在评估Ag NPs对成年雄性白化大鼠的肾脏毒性作用及其潜在的相关机制，同时研究白藜芦醇在改善这些作用方面的可能作用。30只成年白化大鼠被分为5组：对照组、赋形剂组、白藜芦醇组（30mg/kg）、Ag NPs组（300mg/kg）和白藜芦醇+Ag NPs组。治疗经口给药4周。Ag NPs组显示肾脏重量（绝对和相对）降低，肾损伤分子、中性粒细胞明胶酶相关脂质运载蛋白、胱抑素的尿水平以及血肾生物标志物（肌酐、尿素和钾）升高，氧化应激标志物增加而抗氧化标志物减少，血清沉默信息调节因子1（SIRT1）水平降低。还观察到肾组织中白细胞介素1β、肿瘤坏死因子-α和单核细胞趋化蛋白-1基因表达上调，而肾足蛋白和足细胞裂孔素基因表达下调。这些变化与肾小球和肾小管的组织学改变以及胶原纤维面积百分比增加有关。与其他组相比，Ag NPs组中1和紧密连接蛋白-1免疫染色的光密度显著增加。白藜芦醇通过其抗氧化、抗炎和激活SIRT1的作用减轻了所有这些变化，表明其可用作肾脏保护剂。
