Animal Physiology Laboratory, University of Sfax, Faculty of Science, BP1171, 3000 Sfax, Tunisia.
Biofactors. 2011 Nov-Dec;37(6):429-38. doi: 10.1002/biof.178. Epub 2011 Oct 18.
Gibberellic acid (GA₃) has been worldwide used in agriculture as a plant growth regulator. The purpose of this study is to assess the effects of GA₃ on the morphology and the thyroid hormone levels in adult rats and their suckling pups. Animals were given daily 200 ppm GA₃ in drinking water from the 14th day of pregnancy until day 14 after delivery. Compared with a control group, GA₃-treated mothers and pups showed an increase in body and thyroid weights, a decrease in plasma FT₄ and FT₃ levels, which were more pronounced in pups than in their mothers. Thyroid iodine content was also decreased in pups. These biochemical modifications corresponded histologically; the majority of follicles had cubical epithelial cells, which surrounded empty vesicular cavities. Toxicity was objectified by a significant increase in plasma malondialdehyde, protein carbonyls, and advanced oxidation protein products levels in GA₃-treated dams and their suckling pups; while, the activities of superoxide dismutase, catalase, and glutathione peroxidase were decreased in plasma of both dams and their pups. Moreover, a significant decline was observed in plasma glutathione, nonprotein thiols, and vitamin C levels. We conclude that GA₃ treatment affects thyroid function and plasma antioxidant status in adult rats and their progeny.
赤霉素（GA₃）已在世界范围内作为植物生长调节剂应用于农业。本研究旨在评估 GA₃对成年大鼠及其哺乳期幼崽形态和甲状腺激素水平的影响。动物从妊娠第 14 天至分娩后第 14 天每天通过饮用水给予 200 ppm 的 GA₃。与对照组相比，GA₃处理的母亲和幼崽的体重和甲状腺重量增加，血浆 FT₄ 和 FT₃ 水平降低，幼崽比母亲更为明显。幼崽的甲状腺碘含量也减少。这些生化变化在组织学上是对应的；大多数滤泡具有围绕空泡腔的立方上皮细胞。GA₃处理的母鼠及其哺乳期幼崽的血浆丙二醛、蛋白质羰基和高级氧化蛋白产物水平显著增加，客观地体现了毒性；而超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性在母鼠及其幼崽的血浆中均降低。此外，血浆谷胱甘肽、非蛋白巯基和维生素 C 水平也显著下降。我们得出结论，GA₃处理会影响成年大鼠及其后代的甲状腺功能和血浆抗氧化状态。
