Nyambat Batmunkh, Gantuya Sengee, Batuwanthudawe Ranjith, Wijesinghe Pushpa R, Abeysinghe Nihal, Galagoda Geethani, Kirkwood Carl, Bogdanovic-Sakran Nada, Kang Jung Oak, Kilgore Paul E
Division of Translational Research, International Vaccine Institute, Seoul, Korea.
J Infect Dis. 2009 Nov 1;200 Suppl 1:S160-6. doi: 10.1086/605030.
Rotavirus is the most common cause of severe gastroenteritis among children. We conducted hospital-based surveillance to estimate the burden of hospitalizations for rotavirus among children aged <5 years and to describe strain distribution patterns during the 2-year study period.
Children aged <5 years with diarrhea were prospectively enrolled and evaluated by trained pediatricians at representative hospitals in Mongolia and Sri Lanka. Fecal specimens were tested by rotavirus antigen detection enzyme immunoassay. Specimens that tested positive for rotavirus were further characterized to determine the genotype of strains by reverse-transcriptase polymerase chain reaction.
From 1 March 2005 through 28 February 2007, a total of 1277 hospitalized children with diarrhea were enrolled in Mongolia, and 1916 were enrolled in Sri Lanka. Of the 1152 children in Mongolia who had samples tested, 458 (40%) had results positive for rotavirus, and in Sri Lanka, 428 (24%) of 1806 children with samples tested had positive results. G3P[8] was the most common genotype among rotavirus strains in Mongolia (68%) and Sri Lanka (15%).
Rotavirus causes 40% and 24% of hospitalizations for diarrhea among children in Mongolia and Sri Lanka, respectively. Each study site will continue surveillance of rotavirus, and additional laboratory testing will be performed to provide additional information on the distribution of rotavirus strains by G and P genotype.
轮状病毒是儿童严重胃肠炎最常见的病因。我们开展了以医院为基础的监测，以估计5岁以下儿童轮状病毒住院负担，并描述两年研究期间的毒株分布模式。
前瞻性纳入蒙古和斯里兰卡代表性医院5岁以下腹泻儿童，并由经过培训的儿科医生进行评估。粪便标本采用轮状病毒抗原检测酶免疫测定法检测。对轮状病毒检测呈阳性的标本进一步进行鉴定，通过逆转录聚合酶链反应确定毒株的基因型。
2005年3月1日至2007年2月28日，蒙古共纳入1277例腹泻住院儿童，斯里兰卡纳入1916例。在蒙古接受样本检测的1152名儿童中，458名（40%）轮状病毒检测结果呈阳性；在斯里兰卡，1806名接受样本检测的儿童中有428名（24%）检测结果呈阳性。G3P[8]是蒙古（68%）和斯里兰卡（15%）轮状病毒毒株中最常见的基因型。
轮状病毒分别导致蒙古和斯里兰卡40%和24%的儿童腹泻住院病例。每个研究地点将继续监测轮状病毒，并将进行额外的实验室检测，以提供关于按G和P基因型划分的轮状病毒毒株分布的更多信息。
