Mathews Amit, Holland Linda, Yankulov Krassimir
Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1.
FEBS Lett. 2009 Nov 3;583(21):3431-6. doi: 10.1016/j.febslet.2009.10.009. Epub 2009 Oct 9.
ELL-associated protein 30 (EAP30) was initially characterized as a component of the Holo-ELL complex, which contains the elongation factor ELL. Both ELL and Holo-ELL stimulate RNA pol II elongation in vitro. However, ELL and not Holo-ELL inhibits RNA pol II initiation. It is not clear how these two discrete functions of ELL are regulated. Here we report that mini-chromosome maintenance 2 (MCM2) binds to EAP30 and show that MCM2 competes with ELL for binding to EAP30 thus potentially modulating the stability of Holo-ELL.
ELL相关蛋白30（EAP30）最初被鉴定为全酶-ELL复合物的一个组分，该复合物包含延伸因子ELL。ELL和全酶-ELL在体外均能刺激RNA聚合酶II的延伸。然而，抑制RNA聚合酶II起始的是ELL而非全酶-ELL。目前尚不清楚ELL的这两种不同功能是如何被调控的。在此我们报告微小染色体维持蛋白2（MCM2）与EAP30结合，并表明MCM2与ELL竞争结合EAP30，从而可能调节全酶-ELL的稳定性。
