EAP30与ELL之间的相互作用受MCM2调节。

The interaction between EAP30 and ELL is modulated by MCM2.

作者信息

Mathews Amit, Holland Linda, Yankulov Krassimir

机构信息

Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1.

出版信息

FEBS Lett. 2009 Nov 3;583(21):3431-6. doi: 10.1016/j.febslet.2009.10.009. Epub 2009 Oct 9.

DOI:10.1016/j.febslet.2009.10.009

PMID:19819239

Abstract

ELL-associated protein 30 (EAP30) was initially characterized as a component of the Holo-ELL complex, which contains the elongation factor ELL. Both ELL and Holo-ELL stimulate RNA pol II elongation in vitro. However, ELL and not Holo-ELL inhibits RNA pol II initiation. It is not clear how these two discrete functions of ELL are regulated. Here we report that mini-chromosome maintenance 2 (MCM2) binds to EAP30 and show that MCM2 competes with ELL for binding to EAP30 thus potentially modulating the stability of Holo-ELL.

摘要

ELL相关蛋白30（EAP30）最初被鉴定为全酶-ELL复合物的一个组分，该复合物包含延伸因子ELL。ELL和全酶-ELL在体外均能刺激RNA聚合酶II的延伸。然而，抑制RNA聚合酶II起始的是ELL而非全酶-ELL。目前尚不清楚ELL的这两种不同功能是如何被调控的。在此我们报告微小染色体维持蛋白2（MCM2）与EAP30结合，并表明MCM2与ELL竞争结合EAP30，从而可能调节全酶-ELL的稳定性。