Institut National de la Recherche Scientifique - Institut Armand-Frappier, Laval, Quebec, Canada H7V 1B7.
Mar Pollut Bull. 2010 Feb;60(2):291-8. doi: 10.1016/j.marpolbul.2009.09.006. Epub 2009 Oct 12.
Effects of polybrominated diphenyl ethers (PBDEs) on the immune system of marine mammals are poorly understood. One important innate immune function of granulocytes is the respiratory burst which generates reactive oxygen species (ROS) used to kill engulfed microorganisms. The present study investigates in vitro the effects of BDE-47, -99 and -153, on the formation of ROS, on intracellular level of thiols, on activity and efficiency of phagocytosis and on apoptosis in granulocytes of harbour seals. Compounds were tested at four different concentrations ranging from 1.5 to 12 microM. Results showed that ROS levels, thiol levels and phagocytosis were all affected when harbour seal cells were exposed to the highest concentration (12 microM) of PBDE congeners. Apoptosis was not affected by PBDEs. The observed effects were similar in adults, pups and in the 11B7501 cell line of harbour seals.
多溴二苯醚 (PBDEs) 对海洋哺乳动物免疫系统的影响尚未得到充分理解。粒细胞的一个重要固有免疫功能是呼吸爆发，它会产生用于杀死吞噬微生物的活性氧物质 (ROS)。本研究调查了 BDE-47、BDE-99 和 BDE-153 在体外对港湾海豹粒细胞 ROS 形成、细胞内巯基水平、吞噬作用活性和效率以及细胞凋亡的影响。在 1.5 至 12 microM 的四个不同浓度下测试了化合物。结果表明，当港湾海豹细胞暴露于 PBDE 同系物的最高浓度（12 microM）时，ROS 水平、巯基水平和吞噬作用均受到影响。凋亡不受 PBDE 影响。在成年海豹、幼崽和港湾海豹 11B7501 细胞系中观察到的影响相似。
