Sullivan Donna C, Flowers Heather, Rockhold Robin, Herath H M T Bandara, Nanayakkara N P Dhammika
Department of Medicine, Division of Infectious Diseases School of Medicine, University of Mississippi Medical Center, Jackson, Mississippi 39216-4505, USA.
Am J Med Sci. 2009 Oct;338(4):287-91. doi: 10.1097/MAJ.0b013e3181af8270.
We determined the in vitro activity of 9 synthetic fire ant venom alkaloids (+/-)-solenopsin A, (2R, 6R)-solenopsin A, (2S, 6S)-solenopsin B, (+/-)-isosolenopsin A, (2S, 6R)-isosolenopsin A,(2R, 6S)-isosolenopsin A, (+/-)-isosolenopsin B, (2S, 6R)-isosolenopsin B, and (2R, 6S)-isosolenopsin B against 6 species of bacteria (Streptococcus pneumoniae, Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Stenotrophomonas maltophilia, and Pseudomonas aeruginosa).
The minimum inhibitory concentration and minimum bacteriocidal concentration were determined in accordance with the Clinical Laboratory Standards Institute guidelines. Time kill studies used American Type Culture Collection bacterial isolates tested at 5 times the minimum inhibitory concentration.
None of the venom alkaloids inhibited E. coli or P. aeruginosa, whereas all the alkaloids inhibited S. pneumoniae. Only 4 alkaloids inhibited S. pneumoniae, S. aureus, and S. maltophilia. Time-kill kinetics indicates that all 4 active alkaloids had bactericidal activity.
Specific isomers of synthetic fire ant venom alkaloids have antibacterial activity against human pathogens.
我们测定了9种合成火蚁毒液生物碱（±）-索诺普辛A、（2R，6R）-索诺普辛A、（2S，6S）-索诺普辛B、（±）-异索诺普辛A、（2S，6R）-异索诺普辛A、（2R，6S）-异索诺普辛A、（±）-异索诺普辛B、（2S，6R）-异索诺普辛B和（2R，6S）-异索诺普辛B对6种细菌（肺炎链球菌、金黄色葡萄球菌、粪肠球菌、大肠杆菌、嗜麦芽窄食单胞菌和铜绿假单胞菌）的体外活性。
根据临床实验室标准协会的指南测定最低抑菌浓度和最低杀菌浓度。时间杀菌研究使用美国典型培养物保藏中心的细菌分离株，以最低抑菌浓度的5倍进行测试。
没有一种毒液生物碱能抑制大肠杆菌或铜绿假单胞菌，而所有生物碱都能抑制肺炎链球菌。只有4种生物碱能抑制肺炎链球菌、金黄色葡萄球菌和嗜麦芽窄食单胞菌。时间杀菌动力学表明，所有4种活性生物碱都具有杀菌活性。
合成火蚁毒液生物碱的特定异构体对人类病原体具有抗菌活性。
