Costanzi Porrini S, Sciarra A, Sulli N, Piane M, Gualtieri R, Del Porto G
Chair of Medical Genetics, University of Rome La Sapienza, Italy.
Acta Genet Med Gemellol (Roma). 1990;39(4):473-7. doi: 10.1017/s000156600000369x.
The use of DNA restriction fragment length polymorphisms (RFLP) to analyze variable number of tandem repeat (VNTR) sequences dispersed in the human genome, has become a powerful tool for the study of population genetics due to the very substantial polymorphism involved. Because the markers usually employed for twin zygosity determination (such as sex combination, placentation, HLA typing, blood group antigens, etc) may not be uniformly informative, we propose the use of synthetic olygonucleotides, representing VNTR "core" sequences, for the determination of zygosity in twins.
利用DNA限制性片段长度多态性（RFLP）来分析分散在人类基因组中的可变数目串联重复序列（VNTR），由于涉及到非常显著的多态性，已成为群体遗传学研究的有力工具。由于通常用于确定双合子性别的标记（如性别组合、胎盘形成、HLA分型、血型抗原等）可能并非始终具有足够的信息量，我们建议使用代表VNTR“核心”序列的合成寡核苷酸来确定双胞胎的合子性。
