肿瘤相关巨噬细胞是将基于环糊精的纳米粒子递送入神经胶质瘤的主要载体。
Tumor-associated macrophages are predominant carriers of cyclodextrin-based nanoparticles into gliomas.
机构信息
Division of Neurosurgery, City of Hope National Cancer Center, Duarte, California, USA.
出版信息
Nanomedicine. 2010 Apr;6(2):382-90. doi: 10.1016/j.nano.2009.10.001. Epub 2009 Nov 5.
UNLABELLED
The goal of this study was to evaluate the mechanism of cyclodextrin-based nanoparticle (CDP-NP) uptake into a murine glioma model. Using mixed in vitro culture systems, we demonstrated that CDP-NPs were preferentially taken up by BV2 and N9 microglia (MG) cells compared with GL261 glioma cells. Fluorescent microscopy and flow cytometry analysis of intracranial GL261 gliomas confirmed these findings and demonstrated a predominant CDP-NP uptake by macrophages (MPs) and MG within and around the tumor site. Notably, in mice bearing bilateral intracranial tumor, MG and MPs carrying CDP-NPs were able to migrate to the contralateral tumors. In conclusion, these studies better characterize the cellular distribution of CDP-NPs in intracranial tumors and demonstrate that MPs and MG could potentially be used as nanoparticle drug carriers into malignant brain tumors.
FROM THE CLINICAL EDITOR
The goal of this study was to evaluate the mechanism of cyclodextrin-based nanoparticle (CDP-NP) uptake into a murine glioma model. CDP-NP was preferentially taken up microglia (MG) cells as compared to glioma cells. A predominant CDP-NP uptake by macrophages and MG was also shown in and around the tumor site. Macrophages and MG could potentially be used as nanoparticle drug carriers into malignant brain tumors.
目的
本研究旨在评估环糊精纳米颗粒（CDP-NP）进入小鼠脑胶质瘤模型的摄取机制。
方法
采用混合体外培养系统，我们发现 CDP-NP 优先被 BV2 和 N9 小胶质细胞（MG）摄取，而不是 GL261 神经胶质瘤细胞。颅内 GL261 神经胶质瘤的荧光显微镜和流式细胞术分析证实了这些发现，并表明在肿瘤部位内外，CDP-NP 主要被巨噬细胞（MPs）和 MG 摄取。值得注意的是，在双侧颅内肿瘤的小鼠中，携带 CDP-NP 的 MG 和 MPs 能够迁移到对侧肿瘤。
结论
这些研究更好地描述了 CDP-NP 在颅内肿瘤中的细胞分布，并表明 MPs 和 MG 可能可作为纳米药物载体用于恶性脑肿瘤。
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