Zou Lei, Tao Youhua, Payne Gregory, Do Linh, Thomas Tima, Rodriguez Juan, Dou Huanyu
Department of Biomedical Sciences, Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center El Paso, Texas 79905, USA.
Graduate School of Biomedical Sciences, Texas Tech University Health Sciences Center El Paso, Texas 79905, USA.
Oncotarget. 2017 Jan 24;8(4):6564-6578. doi: 10.18632/oncotarget.14169.
Nanoparticles containing mixed lipid monolayer shell, biodegradable polymer core and rabies virus glycoprotein (RVG) peptide as brain targeting ligand, were developed for brain targeted delivery of paclitaxel (PTX) to treat malignant glioma. RVG conjugated PTX loaded NPs (RVG-PTX-NPs) had the desirable size (~140 nm), narrow size distribution and spherical shape. RVG-PTX-NPs showed poor uptake by neurons and selective targeting to the brain tumor associated macrophages (TAMs) with controlled release and tumor specific toxicity. In vivo studies revealed that RVG-PTX-NPs were significant to cross the blood-brain barrier (BBB) and had specific targeting to the brain. Most importantly, RVG-PTX-NPs showed effectiveness for anti-glioma therapy on human glioma of mice model. We concluded that RVG-PTX-NPs provided an effective approach for brain-TAMs targeted delivery for the treatment of glioma.
开发了一种纳米颗粒，其具有混合脂质单层外壳、可生物降解的聚合物核心以及作为脑靶向配体的狂犬病病毒糖蛋白（RVG）肽，用于将紫杉醇（PTX）脑靶向递送至恶性胶质瘤以进行治疗。RVG偶联的载PTX纳米颗粒（RVG-PTX-NPs）具有理想的尺寸（约140nm）、窄尺寸分布和球形形状。RVG-PTX-NPs对神经元的摄取较差，对脑肿瘤相关巨噬细胞（TAM）具有选择性靶向作用，并具有控释和肿瘤特异性毒性。体内研究表明，RVG-PTX-NPs能够有效穿过血脑屏障（BBB）并对脑具有特异性靶向作用。最重要的是，RVG-PTX-NPs在小鼠模型的人胶质瘤抗胶质瘤治疗中显示出有效性。我们得出结论，RVG-PTX-NPs为脑-TAM靶向递送治疗胶质瘤提供了一种有效方法。
