Department of Anesthesiology, The Ninth Hospital, School of Medicine, JiaoTong University, Shanghai, PR China.
Injury. 2011 Jan;42(1):72-7. doi: 10.1016/j.injury.2009.09.027. Epub 2009 Oct 17.
Glutamine (Gln) is the most abundant amino acid in blood and tissue fluids and is considered to be essential in certain catabolic conditions. A series of studies has shown that glutamine can attenuate cytokine release, reduce organ damage and improve survival in a rat model of endotoxaemia. The hypothesis for this rat model study is that pre-treatment with Gln reduces the expression of ICAM-1 and attenuates lung injury induced by intestinal ischaemia-reperfusion (I/R).
Sprague-Dawley rats were randomised into five groups, namely sham group (sham surgery), Gln groups (three different doses) and control group. Lung injury caused by intestinal I/R was evaluated using Evans blue dye concentration and histopathologic examination. The level of myeloperoxidase (MPO) was measured using biochemistry method. The expression of heat shock protein 70 (HSP 70) and ICAM-1 were detected using Western blot and real-time polymerase chain reaction (PCR) methods, respectively.
Compared with the control group, rats pre-treated with Gln before intestinal I/R demonstrated decreased Evans Blue content and MPO activities in lung tissue, reduced the expression of ICAM-1, attenuated lung injury evidenced by pathological change compared with lactated Ringer pre-treated rats. Gln administration increased HSP 70 mRNA and protein expression in lung tissue compared with control group.
Ischaemia-reperfusion injury increases the expression of ICAM-1 in the lung. This may contribute to the migration, accumulation and activation of neutrophils. Pre-treatment with Gln attenuates rat lung injury and reduces ICAM-1 expression.
谷氨酰胺（Gln）是血液和组织液中含量最丰富的氨基酸，被认为是某些分解代谢状态下的必需氨基酸。一系列研究表明，谷氨酰胺可以减弱细胞因子的释放，减轻器官损伤，并提高内毒素血症大鼠模型的存活率。本大鼠模型研究的假设是，谷氨酰胺预处理可降低 ICAM-1 的表达，并减轻肠缺血再灌注（I/R）引起的肺损伤。
将 Sprague-Dawley 大鼠随机分为五组，即假手术组（假手术）、Gln 组（三个不同剂量）和对照组。通过 Evans 蓝染料浓度和组织病理学检查评估肠 I/R 引起的肺损伤。采用生化方法测定髓过氧化物酶（MPO）水平。采用 Western blot 和实时聚合酶链反应（PCR）方法分别检测热休克蛋白 70（HSP 70）和细胞间黏附分子-1（ICAM-1）的表达。
与对照组相比，肠 I/R 前用 Gln 预处理的大鼠肺组织 Evans Blue 含量和 MPO 活性降低，ICAM-1 表达减少，与用乳酸林格氏液预处理的大鼠相比，肺损伤减轻。与对照组相比，Gln 给药组大鼠肺组织 HSP 70 mRNA 和蛋白表达增加。
缺血再灌注损伤增加了肺组织中 ICAM-1 的表达。这可能有助于中性粒细胞的迁移、聚集和激活。Gln 预处理可减轻大鼠肺损伤并降低 ICAM-1 的表达。
