Central Veterinary Research Laboratory, P.O. Box 8067, Al Amarat, Khartoum, Sudan.
Acta Trop. 2010 Feb;113(2):129-33. doi: 10.1016/j.actatropica.2009.10.005. Epub 2009 Oct 17.
This study aimed to investigate the occurrence of respiratory syncytial virus (RSV) infections in camels in Sudan. A total of 272 camel lung specimens showing pneumonia were collected from slaughter houses at four different areas in Sudan, additionally 8 specimens were collected from outbreaks of respiratory infection in camels. Using sandwich ELISA kits for RSV antigen detection 4 out of 280 tested lungs (1.4%) were positive, all were from Central Sudan (Tambool slaughter house). FAT was used to confirm the ELISA positives. Polymerase chain reaction RT/PCR was applied for the detection of RSV genome in camel lungs; 1 out of 4 ELISA positives was positive by RT/PCR. Using indirect ELISA kits 135 out of 495 (27.3%) camel sera showed antibodies to RSV, highest prevalence was observed in Western (33.5%) then Central (31.6%) and Eastern Sudan (23.5%). Based on the manufacturer specified calculations for OD readings, most of positive sera (90/135) were low reactive (1+). This is the first report for the detection of RSV antigen, genome and antibody in camels in Sudan.
本研究旨在调查苏丹骆驼呼吸道合胞病毒 (RSV) 感染的发生情况。从苏丹四个不同地区的屠宰场采集了 272 份表现为肺炎的骆驼肺标本，此外还从骆驼呼吸道感染爆发中采集了 8 份标本。使用 RSV 抗原检测的夹心 ELISA 试剂盒，在 280 份检测的肺组织中，有 4 份（1.4%）为阳性，均来自苏丹中部（Tambool 屠宰场）。FAT 用于确认 ELISA 阳性结果。应用 RT/PCR 检测骆驼肺中的 RSV 基因组，在 4 份 ELISA 阳性中，有 1 份通过 RT/PCR 呈阳性。使用间接 ELISA 试剂盒，在 495 份骆驼血清中，有 135 份（27.3%）显示出针对 RSV 的抗体，其中西部（33.5%）苏丹的阳性率最高，其次是中部（31.6%）和东部（23.5%）。根据制造商指定的 OD 读数计算，大多数阳性血清（90/135）的反应性较低（1+）。这是在苏丹首次报道骆驼中 RSV 抗原、基因组和抗体的检测。
