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本文引用的文献

1
Messenger RNA export from the nucleus: a series of molecular wardrobe changes.mRNA 从细胞核输出：一系列分子换装。
Traffic. 2009 Sep;10(9):1199-208. doi: 10.1111/j.1600-0854.2009.00944.x. Epub 2009 Jun 27.
2
Purification and visualization of influenza a viral ribonucleoprotein complexes.甲型流感病毒核糖核蛋白复合体的纯化与可视化
J Vis Exp. 2009 Feb 9(24):1105. doi: 10.3791/1105.
3
Nuclear transport factor directs localization of protein synthesis during mitosis.核转运因子在有丝分裂期间指导蛋白质合成的定位。
Nat Cell Biol. 2009 Mar;11(3):350-6. doi: 10.1038/ncb1844. Epub 2009 Feb 8.
4
Functional significance of the interaction between the mRNA-binding protein, Nab2, and the nuclear pore-associated protein, Mlp1, in mRNA export.mRNA 结合蛋白 Nab2 与核孔相关蛋白 Mlp1 之间的相互作用在 mRNA 输出中的功能意义。
J Biol Chem. 2008 Oct 3;283(40):27130-43. doi: 10.1074/jbc.M803649200. Epub 2008 Aug 5.
5
GraFix: sample preparation for single-particle electron cryomicroscopy.GraFix：用于单颗粒冷冻电子显微镜的样品制备方法
Nat Methods. 2008 Jan;5(1):53-5. doi: 10.1038/nmeth1139. Epub 2007 Dec 23.
6
The DEAD-box protein Dbp5 controls mRNA export by triggering specific RNA:protein remodeling events.DEAD盒蛋白Dbp5通过触发特定的RNA：蛋白质重塑事件来控制mRNA输出。
Mol Cell. 2007 Dec 14;28(5):850-9. doi: 10.1016/j.molcel.2007.09.019.
7
Gene expression profiling by massively parallel sequencing.通过大规模平行测序进行基因表达谱分析。
Genome Res. 2008 Jan;18(1):172-7. doi: 10.1101/gr.6984908. Epub 2007 Nov 21.
8
Comprehensive analysis of diverse ribonucleoprotein complexes.多种核糖核蛋白复合物的综合分析
Nat Methods. 2007 Nov;4(11):951-6. doi: 10.1038/nmeth1101. Epub 2007 Oct 7.
9
Exporting RNA from the nucleus to the cytoplasm.将RNA从细胞核输出到细胞质。
Nat Rev Mol Cell Biol. 2007 Oct;8(10):761-73. doi: 10.1038/nrm2255.
10
Recognition of polyadenosine RNA by zinc finger proteins.锌指蛋白对多聚腺苷酸RNA的识别。
Proc Natl Acad Sci U S A. 2007 Jul 24;104(30):12306-11. doi: 10.1073/pnas.0701244104. Epub 2007 Jul 13.

核多聚 A 结合蛋白 Nab2 的纯化揭示了其与酵母转录组的关联及其信使核糖核蛋白核心结构。

Purification of nuclear poly(A)-binding protein Nab2 reveals association with the yeast transcriptome and a messenger ribonucleoprotein core structure.

机构信息

Biochemie Zentrum der Universität Heidelberg (BZH), Im Neuenheimer Feld 307, D-69120 Heidelberg, Germany.

出版信息

J Biol Chem. 2009 Dec 11;284(50):34911-7. doi: 10.1074/jbc.M109.062034. Epub 2009 Oct 19.

DOI:10.1074/jbc.M109.062034
PMID:19840948
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2787353/
Abstract

Nascent mRNAs produced by transcription in the nucleus are subsequently processed and packaged into mRNA ribonucleoprotein particles (messenger ribonucleoproteins (mRNPs)) before export to the cytoplasm. Here, we have used the poly(A)-binding protein Nab2 to isolate mRNPs from yeast under conditions that preserve mRNA integrity. Upon Nab2-tandem affinity purification, several mRNA export factors were co-enriched (Yra1, Mex67, THO-TREX) that were present in mRNPs of different size and mRNA length. High-throughput sequencing of the co-precipitated RNAs indicated that Nab2 is associated with the bulk of yeast transcripts with no specificity for different mRNA classes. Electron microscopy revealed that many of the mRNPs have a characteristic elongated structure. Our data suggest that mRNPs, although associated with different mRNAs, have a unifying core structure.

摘要

在细胞核中转录产生的新生 mRNA 随后经过加工并包装成 mRNA 核糖核蛋白颗粒（信使核糖核蛋白（mRNPs）），然后输出到细胞质。在这里，我们使用多聚（A）结合蛋白 Nab2 在保持 mRNA 完整性的条件下从酵母中分离 mRNPs。在 Nab2 串联亲和纯化后，几种 mRNA 输出因子（Yra1、Mex67、THO-TREX）被共富集，这些因子存在于不同大小和 mRNA 长度的 mRNPs 中。共沉淀 RNA 的高通量测序表明，Nab2 与大量酵母转录物相关，而对不同的 mRNA 类别没有特异性。电子显微镜显示，许多 mRNPs 具有特征性的细长结构。我们的数据表明，mRNPs 尽管与不同的 mRNA 相关，但具有统一的核心结构。