Department of Chemistry, McGill University, 801 Sherbrooke St. W., #322, Montreal, Quebec, Canada, H3A 2K6.
Trends Biochem Sci. 2009 Dec;34(12):601-11. doi: 10.1016/j.tibs.2009.07.004. Epub 2009 Oct 19.
Biological macromolecules are highly flexible and continually undergo conformational fluctuations on a broad spectrum of timescales. It has long been recognized that dynamics have an important role in the action of these molecules. However, the relationship between molecular function and motion is extremely challenging to delineate, because the conformational space available to macromolecules is vast and the relevant excursions can be infrequent and short-lived. Recent advances in solution nuclear magnetic resonance (NMR) spectroscopy permit biomolecular dynamics to be observed with unprecedented detail. Applications of these new NMR techniques to the study of fundamental processes such as binding and catalysis have provided new insights into how living systems operate at an atomic level.
生物大分子具有高度的灵活性，并在广泛的时间尺度上不断经历构象波动。长期以来，人们一直认识到，动力学在这些分子的作用中起着重要的作用。然而，分子功能和运动之间的关系极难描绘，因为大分子可用的构象空间是巨大的，并且相关的扩展可能很少且短暂。溶液核磁共振（NMR）光谱学的最新进展使得能够以前所未有的细节观察生物分子动力学。这些新的 NMR 技术在结合和催化等基本过程的研究中的应用，为了解生命系统在原子水平上的运作方式提供了新的见解。
