Medical School, Department of Pathology, Hacettepe University, Sihhiye, Ankara, Turkey.
Int J Gynecol Pathol. 2009 Nov;28(6):541-8. doi: 10.1097/PGP.0b013e3181aaba0d.
The main aim of the study is to describe the human papillomavirus (HPV) type-specific distribution in invasive cervical cancer (ICC) specimens from Turkey. Paraffin-embedded ICC specimens were identified from the histopathologic archives of the Hacettepe University Medical School in Turkey. HPV detection was carried out through amplification of HPV DNA by a SPF-10 broad-spectrum primer polymerase chain reaction and subsequently followed by DNA enzyme immunoassay and genotyping by LiPA25 (version 1). Two hundred seventy-seven ICC cases diagnosed between 1993 and 2004 were retrieved. After histologic evaluation and human beta-globin gene analysis for sample quality, 248 cases were considered suitable for HPV/DNA testing. HPV prevalence was 93.5% (232/248; 95% confidence interval: 90.5%-96.6%). The five most common HPV types identified as single types among HPV-positive cases were HPV16 (64.7%), HPV18 (9.9%), HPV45 (9.9%), HPV31 (3.0%), and HPV33 (2.2%). The study shows that in Turkey, HPV16/HPV18 accounted for 75.4% (95% confidence interval: 69.9%-81.0%) of HPV-positive ICC cases. This information is essential to evaluate the potential impact of the HPV vaccines in the country.
本研究的主要目的是描述来自土耳其的浸润性宫颈癌（ICC）标本中人乳头瘤病毒（HPV）的型别特异性分布。从土耳其哈塞特佩大学医学院的组织病理学档案中鉴定出石蜡包埋的 ICC 标本。通过 SPF-10 广谱引物聚合酶链反应扩增 HPV DNA 进行 HPV 检测，随后进行 DNA 酶免疫测定和 LiPA25（版本 1）基因分型。检索了 1993 年至 2004 年间诊断的 277 例 ICC 病例。经过组织学评估和人β-珠蛋白基因分析以评估样本质量后，认为 248 例适合 HPV/DNA 检测。HPV 流行率为 93.5%（232/248；95%置信区间：90.5%-96.6%）。在 HPV 阳性病例中，作为单一类型鉴定出的五种最常见的 HPV 类型是 HPV16（64.7%）、HPV18（9.9%）、HPV45（9.9%）、HPV31（3.0%）和 HPV33（2.2%）。该研究表明，在土耳其，HPV16/HPV18 占 HPV 阳性 ICC 病例的 75.4%（95%置信区间：69.9%-81.0%）。这些信息对于评估 HPV 疫苗在该国的潜在影响至关重要。
