Jha Rashmi, Rizvi Syed Ibrahim
Department of Biochemistry, University of Allahabad, India.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2009 Sep;153(3):195-8. doi: 10.5507/bp.2009.032.
Oxidative stress hypothesis offers a mechanism for the aging process and its involvement in other pathologies such as diabetes and neurodegenerative diseases like Alzheimer. AChE activity in erythrocytes may be considered as a marker of central cholinergic status. The present study was undertaken to (i) determine the activity of erythrocyte AChE as a function of human process (ii) correlate AChE activity with oxidative stress during human aging.
Blood was collected from healthy subjects (n = 37) 22-82 years. Erythrocyte AChE activity, MDA and plasma antioxidant capacity in terms of FRAP was measured spectrophotometrically.
There was a marked decrease in AChE activity with increasing age. The reduction in activity of AChE correlated well with increased lipid peroxidation and a decrease in FRAP values.
Decreased antioxidant defense, and alteration in membrane rheology during aging process both may contribute towards decreased activity of AChE in erythrocyte membrane. This finding may help in explaining the neuronal complications taking place under conditions of oxidative stress, aging, and dementia.
氧化应激假说为衰老过程及其在其他病理状况（如糖尿病和阿尔茨海默病等神经退行性疾病）中的作用提供了一种机制。红细胞中的乙酰胆碱酯酶（AChE）活性可被视为中枢胆碱能状态的标志物。本研究旨在（i）确定红细胞AChE活性随人类衰老过程的变化情况，（ii）将AChE活性与人类衰老过程中的氧化应激相关联。
采集了年龄在22至82岁之间的37名健康受试者的血液。用分光光度法测定红细胞AChE活性、丙二醛（MDA）以及血浆抗氧化能力（以铁还原抗氧化能力测定法（FRAP）表示）。
随着年龄增长，AChE活性显著降低。AChE活性的降低与脂质过氧化增加以及FRAP值降低密切相关。
衰老过程中抗氧化防御能力下降以及膜流变学改变，两者可能共同导致红细胞膜中AChE活性降低。这一发现可能有助于解释在氧化应激、衰老和痴呆等情况下发生的神经元并发症。
