Institute for Clinical and Experimental Surgery, University of Saarland, Homburg, Saarland, Germany.
Langenbecks Arch Surg. 2010 Jan;395(1):33-40. doi: 10.1007/s00423-009-0558-9.
The purpose of this study is to determine gender-specific differences in the development of necrosis in persistent ischemic tissue and to analyze whether differences are due to gender-specific loss of vascular reactivity or change in ischemic tolerance.
Hairless mice (skh-1) of both genders were assigned to three groups of adolescent, adult, and senescent age. Critical ischemia was induced by transection of the two distal pedicles of the animal's ear. Microcirculation was assessed over a 5-day period using intravital epifluorescence microscopy. Tissue necrosis, blood flow, functional capillary density (FCD), red blood cell (RBC) velocity, and capillary diameter were analyzed.
Induction of persistent ischemia caused an age-dependent demarcation of nonperfused flap tissue. Adult and senescent females developed markedly more necrosis than age-matched males (49 +/-1% vs. 37 +/-3% and 53 +/- 3% vs. 44 +/- 2%, respectively; p<0.05), whereas no gender-specific difference in flap necrosis was observed in adolescent animals (31 +/- 2% vs. 33 +/- 3%). Gender did not affect the amount of microcirculatory dysfunction in the flap. Thus, age-matched females and males exhibited a comparable decrease of FCD, RBC velocity, and capillary dilatory response.
Both age and female gender may predispose for an increased susceptibility to develop ischemic tissue necrosis. The increased necrosis in female animals does not apply to an aggravated microvascular dysfunction, but rather to a reduced ischemic tissue tolerance.
本研究旨在确定持续性缺血组织坏死在性别特异性方面的差异，并分析这些差异是否归因于血管反应性的性别特异性丧失或缺血耐受性的变化。
将两种性别的无毛小鼠（skh-1）分配到青少年、成年和老年三个年龄组。通过切断动物耳朵的两个远端蒂来诱导临界缺血。在 5 天的时间内，使用活体荧光显微镜评估微循环。分析组织坏死、血流、功能毛细血管密度（FCD）、红细胞（RBC）速度和毛细血管直径。
持续缺血的诱导导致非灌注瓣组织出现年龄依赖性界限。成年和老年雌性比年龄匹配的雄性发展出明显更多的坏死（49 +/-1%对 37 +/-3%和 53 +/- 3%对 44 +/- 2%；p<0.05），而青少年动物中没有性别特异性的瓣坏死差异（31 +/- 2%对 33 +/- 3%）。性别对瓣的微循环功能障碍没有影响。因此，年龄匹配的雌性和雄性表现出相似的 FCD、RBC 速度和毛细血管扩张反应的减少。
年龄和女性性别都可能导致缺血组织坏死的易感性增加。雌性动物中增加的坏死并不适用于加重的微血管功能障碍，而是缺血组织耐受性的降低。
