Bilge Uysal Elif, Ozdemir Mehmet, Baykan Mahmut
Konya Eğitim ve Araştirma Hastanesi, Mikrobiyoloji ve Klinik Mikrobiyoloji, Konya, Turkey.
Turkiye Parazitol Derg. 2009;33(3):195-8.
Hydatidosis is an endemic illness in many regions of world. Several serodiagnostic techniques have been evaluated for the diagnosis of cystic hydatid disease caused by Echinococcus granulosus. The aim of this study was to assess the efficiency of the commercial fluorescent antibody test (IFAT), indirect hemaglutination test (IHAT) and in-house IFA tests. For this purpose sera from one hundred patients who had been given a diagnosis of hydatid cyst by surgery were used. In-house IFA was developed using the germinal membrane of Echinococcus granulosus and sera were studied by the three different methods. As a result, the specificity of commercial IFA, IHA and in-house IFA was found to be 100% and sensitivities of these tests were 87.7%, 74.6% and 83.3% respectively. In conclusion, in-house IFA test is a useful and cost effective but difficult test to prepare for the routine laboratory.
包虫病是世界许多地区的一种地方病。已经评估了几种血清学诊断技术用于诊断由细粒棘球绦虫引起的囊型包虫病。本研究的目的是评估商业荧光抗体试验（IFAT）、间接血凝试验（IHAT）和自制免疫荧光试验（IFA）的效率。为此，使用了100例经手术诊断为包虫囊肿的患者的血清。利用细粒棘球绦虫的生发膜开发了自制IFA，并通过三种不同方法对血清进行研究。结果发现，商业IFA、IHA和自制IFA的特异性均为100%，这些试验的敏感性分别为87.7%、74.6%和83.3%。总之，自制IFA试验对于常规实验室来说是一种有用且具有成本效益但制备困难的试验。
