Xu Shi-San, Ni Fang, Luo Da-Min
School of Life Sciences, Xiamen University, Xiamen.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2009 Jun;27(3):248-50.
A total of 1,277 ESTs of Angiostrongylus cantonensis were downloaded from GenBank and analyzed with BlastX. SignalP V3.0 analysis was applied to predict potential putative antigen or allergen relative proteins with N-terminal secreted signal peptides or signal anchors. BlastX analysis showed that there were 614 ESTs scored more than 100, of which 14 were identical with A. cantonensis, 60 ESTs did not match any proteins in the databases. The identified 614 ESTs could be grouped into 10 categories, 80 ESTs expressed 22 antigen or allergen relative proteins, in which 12 had N-terminal secreted signal peptides and 3 had signal anchors.
从GenBank下载了总共1277条广州管圆线虫的EST序列，并使用BlastX进行分析。应用SignalP V3.0分析来预测具有N端分泌信号肽或信号锚的潜在推定抗原或过敏原相关蛋白。BlastX分析表明，有614条EST得分超过100，其中14条与广州管圆线虫相同，60条EST在数据库中未匹配到任何蛋白质。鉴定出的614条EST可分为10类，80条EST表达了22种抗原或过敏原相关蛋白，其中12种具有N端分泌信号肽，3种具有信号锚。
