Chang Shih-Hsin, Tang Petrus, Wang Lian-Chen
Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Tao-Yuan 33332, Taiwan.
Mol Biochem Parasitol. 2011 Sep;179(1):47-50. doi: 10.1016/j.molbiopara.2011.05.005. Epub 2011 May 20.
To investigate the pepsin-activated infective (third-stage) larvae of Angiostrongylus cantonensis at the transcriptomic level, 1496 ESTs were generated from a cDNA library and clustered into 161 contigs and 757 singletons. Among these unigenes, 54.5% had significant sequence homology with known proteins. The most abundantly expressed transcripts were cathepsin B-like cysteine protease 1 and 2, metalloprotease I, metalloprotease 1 precursor, and extracellular superoxide dismutase. Protein complex was the most common Gene Ontology classification within the 'cellular component' category, embryonic development ending in birth or egg hatching within 'biological process', and protein binding within 'molecular function'. Moreover, 280 clusters were mapped to 158 KEGG pathways and 134 had unique EC numbers. These findings suggest that treatment with pepsin-HCl not only digests the tissues of the snail host but also activates the infective larvae.
为了在转录组水平上研究经胃蛋白酶激活的广州管圆线虫感染性（第三期）幼虫，从一个cDNA文库中生成了1496个EST，并将其聚类为161个重叠群和757个单拷贝序列。在这些单基因中，54.5%与已知蛋白质具有显著的序列同源性。表达量最高的转录本是组织蛋白酶B样半胱氨酸蛋白酶1和2、金属蛋白酶I、金属蛋白酶1前体和细胞外超氧化物歧化酶。在“细胞成分”类别中，蛋白质复合体是最常见的基因本体分类；在“生物学过程”中，以出生或卵孵化结束的胚胎发育是最常见的分类；在“分子功能”中，蛋白质结合是最常见的分类。此外，280个聚类被映射到158条KEGG通路，134个具有独特的酶委员会编号。这些发现表明，用胃蛋白酶-盐酸处理不仅能消化蜗牛宿主的组织，还能激活感染性幼虫。
