Langenhan Tobias, Prömel Simone, Mestek Lamia, Esmaeili Behrooz, Waller-Evans Helen, Hennig Christian, Kohara Yuji, Avery Leon, Vakonakis Ioannis, Schnabel Ralf, Russ Andreas P
Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK.
Dev Cell. 2009 Oct;17(4):494-504. doi: 10.1016/j.devcel.2009.08.008.
Understanding the mechanisms that coordinate the orientation of cell division planes during embryogenesis and morphogenesis is a fundamental problem in developmental biology. Here we show that the orphan receptor lat-1, a homolog of vertebrate latrophilins, plays an essential role in the establishment of tissue polarity in the C. elegans embryo. We provide evidence that lat-1 is required for the alignment of cell division planes to the anterior-posterior axis and acts in parallel to known polarity and morphogenesis signals. lat-1 is a member of the Adhesion-GPCR protein family and is structurally related to flamingo/CELSR, an essential component of the planar cell polarity pathway. We dissect the molecular requirements of lat-1 signaling and implicate lat-1 in an anterior-posterior tissue polarity pathway in the premorphogenesis stage of C. elegans development.
理解在胚胎发生和形态发生过程中协调细胞分裂平面方向的机制是发育生物学中的一个基本问题。在此我们表明，孤儿受体lat-1（脊椎动物latrophilins的同源物）在秀丽隐杆线虫胚胎的组织极性建立中起关键作用。我们提供的证据表明，lat-1是细胞分裂平面与前后轴对齐所必需的，并且与已知的极性和形态发生信号平行发挥作用。lat-1是粘附GPCR蛋白家族的成员，在结构上与flamingo/CELSR相关，后者是平面细胞极性通路的重要组成部分。我们剖析了lat-1信号传导的分子需求，并表明lat-1参与了秀丽隐杆线虫发育的形态发生前期阶段的前后组织极性通路。
