Independent Junior Research Group Molecular Biology of Archaea, Max-Planck-Institute for Terrestrial Microbiology, Karl-von-Frisch-Strasse, 35043 Marburg, Germany.
J Bacteriol. 2010 Jan;192(1):104-10. doi: 10.1128/JB.01061-09.
Attachment of microorganisms to surfaces is a prerequisite for colonization and biofilm formation. The hyperthermophilic crenarchaeote Sulfolobus solfataricus was able to attach to a variety of surfaces, such as glass, mica, pyrite, and carbon-coated gold grids. Deletion mutant analysis showed that for initial attachment the presence of flagella and pili is essential. Attached cells produced extracellular polysaccharides containing mannose, galactose, and N-acetylglucosamine. Genes possibly involved in the production of the extracellular polysaccharides were identified.
微生物附着在表面是其定殖和生物膜形成的前提。嗜热古菌嗜酸硫磺酸菌能够附着在各种表面上,如玻璃、云母、黄铁矿和碳涂层的金网格。缺失突变体分析表明,最初的附着需要鞭毛和菌毛的存在。附着的细胞产生含有甘露糖、半乳糖和 N-乙酰葡萄糖胺的细胞外多糖。鉴定了可能参与细胞外多糖产生的基因。