冻干粉犬精子显微注射入卵母细胞的原核形成。
Pronuclear formation of freeze-dried canine spermatozoa microinjected into mouse oocytes.
机构信息
Department of Food Production Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan.
出版信息
J Assist Reprod Genet. 2009 Sep-Oct;26(9-10):531-6. doi: 10.1007/s10815-009-9358-y. Epub 2009 Oct 24.
Abstract
PURPOSE
The aim of the present study was to investigate the fertilizing capacity of fresh, frozen-thawed and freeze-dried canine spermatozoa.
METHODS
After canine spermatozoa were injected into mouse oocytes, the rates of oocyte activation, male pronuclear formation and chromosomal aberrations were investigated.
RESULTS
The rates of oocyte activation were comparable (90.6-100%), no matter the sperm type injected. The percentage of male pronuclear formation was higher (P < 0.001) in the freeze-dried spermatozoa (92.3%) than the fresh (61.5%) and frozen-thawed (69.2%) spermatozoa. However, the chromosomal damage in the oocytes injected with freeze-dried spermatozoa was higher (72.9%: P < 0.001) than with fresh (26.9%) and frozen-thawed (21.4%) spermatozoa.
CONCLUSIONS
These data indicate using mouse oocytes that freeze-dried canine spermatozoa may potentially fertilize canine oocytes although chromosomal damage is frequently generated.
摘要
目的
本研究旨在探讨新鲜、冷冻解冻和冻干犬精子的受精能力。
方法
将犬精子注入小鼠卵母细胞后，研究卵母细胞激活、雄性原核形成和染色体异常的情况。
结果
无论注射的精子类型如何，卵母细胞激活率相当（90.6-100%）。与新鲜（61.5%）和冷冻解冻（69.2%）精子相比，冻干精子（92.3%）的雄性原核形成率更高（P < 0.001）。然而，与新鲜（26.9%）和冷冻解冻（21.4%）精子相比，用冻干精子注射的卵母细胞中的染色体损伤更高（72.9%：P < 0.001）。
结论
这些数据表明，使用小鼠卵母细胞，尽管经常产生染色体损伤，但冻干犬精子可能具有潜在的受精犬卵母细胞的能力。
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